We narrowed to 2,587 results for: PGK

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pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239747

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3780_pGEEC587_pShip-CMV(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239749

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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EW209 PGK-ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)

Plasmid

#236135

Purpose

Plasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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EW196 PGK-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

Plasmid

#236132

Purpose

Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW415 PGK-ZNF35(5-8)-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)

Plasmid

#236136

Purpose

Plasmid encoding the ZNF35/ZNFIKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35/IKZF1(2-3)-deimmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)

Plasmid

#233378

Purpose

PGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in between

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-ADAR2dd

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

PGK

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205544

Purpose

Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

Plasmid

#205543

Purpose

Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-Myc

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

Plasmid

#179440

Purpose

Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-HDAC4

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTopo RE3 Donor pLox hPGK-Puro pA pLox

Plasmid

#171050

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-196a-2SM-PGK-GFP-Blast

Plasmid

#31512

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196a2 (Mir196a-2 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187951

Purpose

FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69546

Purpose

Tet-On system expressing MyoD-2A-GFP-2xNLS.

Depositor

Charles Gersbach

Inserts

eGFP
Myod1 (Myod1 Mouse)

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Mutation

Removed stop codon to fuse it to the 2A-eGFPnls i…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

Plasmid

#84777

Purpose

Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)

Use

Lentiviral

Promoter

pTIGHT

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

Plasmid

#223213

Purpose

Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

Depositor

Wendell Lim

Article

Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

Insert

TGFb1 active - membrane (TGFB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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