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Plasmid#165047PurposeIntegrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…PromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike3
Plasmid#136950PurposePlasmid for the In Vitro transcription of DiySpike3DepositorInsertDiySpike3
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike1d1
Plasmid#136951PurposePlasmid for the In Vitro transcription of DiySpike1d1DepositorInsertDiySpike1d1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike1d2
Plasmid#136952PurposePlasmid for the In Vitro transcription of DiySpike1d2DepositorInsertDiySpike1d2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike1d3
Plasmid#136953PurposePlasmid for the In Vitro transcription of DiySpike1d3DepositorInsertDiySpike1d3
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike1d4
Plasmid#136954PurposePlasmid for the In Vitro transcription of DiySpike1d4DepositorInsertDiySpike1d4
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d1
Plasmid#136955PurposePlasmid for the In Vitro transcription of DiySpike2d1DepositorInsertDiySpike2d1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d2
Plasmid#136956PurposePlasmid for the In Vitro transcription of DiySpike2d2DepositorInsertDiySpike2d2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d3
Plasmid#136957PurposePlasmid for the In Vitro transcription of DiySpike2d3DepositorInsertDiySpike2d3
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2d4
Plasmid#136958PurposePlasmid for the In Vitro transcription of DiySpike2d4DepositorInsertDiySpike2d4
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
UseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas28
Plasmid#82385PurposesgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23108Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas27
Plasmid#82384PurposesgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23117Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD06 K. lactis telomerase RNA hairpin
Plasmid#61895PurposePlasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)DepositorInsertTelomerase RNA hairpin (nt 229-273)
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.G2530AAAC
Plasmid#26190DepositorInsert23S ribosomal RNA fragment (modified)
UseTagsExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…PromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Blast
Plasmid#227257PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only