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We narrowed to 5,014 results for: Streptococcus

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Showing: 1421 - 1440 of 5014 results

  1. pCSD_SpCas9MT3-FKBP_SL

    Plasmid
    #107302
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107302-plasmid-map-sequence-id-204632

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  2. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid
    #107305
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2x NLS and FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107305-plasmid-map-sequence-id-204646

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  3. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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  10. pFW11-ffluc

    Plasmid
    #128487
    Purpose
    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging
    Depositor
    Thomas Proft
    Publication
    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    firefly luciferase gene
    Use
    Allelic replacement vector
    Tags
    spy0535 downstream flanking region and spy0535 up…
    Promoter
    synthetic
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    128487-plasmid-map-sequence-id-266752

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  11. pBBR1-P2-SpCas9

    Plasmid
    #232354
    Purpose
    The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7.
    Depositor
    Yujie Cai
    Publication
    Gou et al Biotechnol Bioeng. 2025 May;122(5):1233-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sacB
    Use
    CRISPR
    Promoter
    promoter P2 from S. marcescens HBQA7
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232354-plasmid-map-sequence-id-485173

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  20. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

    Plasmid
    #101217
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…
    Promoter
    T7
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101217-plasmid-map-sequence-id-197215

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Showing: 1421 - 1440 of 5014 results