We narrowed to 36,226 results for: des.2

1. pCxcl1-Firefly-2-Tbx5-sites

   Plasmid

   #177833

   Purpose

   Luciferase reporter for mouse Cxcl1 with two Tbx5 consensus binding sites

   Depositor

   Alexander Ruthenburg

   Article

   Hall et al bioRxiv 2021.12.23.474040

   Insert

   Luciferase
   

   Use

   Luciferase

   Promoter

   Cxcl1

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-6XHis-human SUMO 2

   Plasmid

   #133771

   Purpose

   express 6Xhis- SUMO 2 protein

   Depositor

   Martine Roussel

   Article

   Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   SUMO 2 (SUMO2 Human)
   

   Tags

   6XHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEnt L1L3 EF1a-tTA-2

   Plasmid

   #26803

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Elongation Factor 1 alpha promoter and tTA transgene
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/Myc-His(-)-HSulf-2

   Plasmid

   #13004

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 (SULF2 Human)
   

   Tags

   6x His and Myc

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-pLKO-FOXM1-shRNA-#2

   Plasmid

   #89499

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   H1/TO

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLKO.1-SMG6-CDS-2

   Plasmid

   #136047

   Purpose

   SMG6 shRNA (Targeting CDS #2) inserted into the PLKO.1 plasmid (AAGGAGTTCCAGGTGTTACTG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   SMG6 (SMG6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Myc-His(-)-MSulf-2

   Plasmid

   #13008

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 2 (Sulf2 Mouse)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 Rheb1 shRNA #2

   Plasmid

   #26626

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Rheb1 shRNA (RHEB Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

9. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 RagD shRNA #2

    Plasmid

    #30322

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagD shRNA (RRAGD Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Expression

    Worm

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-ef1a-DIO-eGCaMP(2+)

    Plasmid

    #201150

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP_2+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 RagB shRNA #2

    Plasmid

    #26628

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagB shRNA (RRAGB Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pGEX6-2 SNAP-Syk tSH2

    Plasmid

    #113028

    Purpose

    for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    SNAP-Syk tSH2
    

    Tags

    SNAP

    Expression

    Bacterial

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBB HA BIR 2-3

    Plasmid

    #25691

    Depositor

    Colin Duckett

    Article

    Lewis et al J Biol Chem. 2004 Mar 5. 279(10):9023-9.

    Insert

    XIAP (XIAP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    amino acid 124RDH-LEE350 (BIR 2&3)

    Available Since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pSUPER-retro-puro-shNup133-2

    Plasmid

    #87333

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK-2-nMyc-m2_3'UTR

    Plasmid

    #31860

    Depositor

    Robert Blelloch

    Article

    Melton et al Nature. 2010 Feb 4;463(7281):621-6. Epub

    Insert

    nMyc mutant-1 3'UTR
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Second Let-7c Seed changed from TACCTC to TAACGC

    Promoter

    SV40

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-H11r1-2-DD-Cas9

    Plasmid

    #85978

    Purpose

    Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    DD-SpCas9
    

    Use

    CRISPR

    Tags

    FKBP12 L106P DD

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. p8390 LentiCRISPRv2 Neo sgNT-2

    Plasmid

    #221650

    Purpose

    Expression of non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    non-targeting sgNT-2
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGTag Hygro EGFP nLAP -2

    Plasmid

    #194322

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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