We narrowed to 32,671 results for: eng

1. pGGB_RT_Venus_li

   Plasmid

   #136966

   Purpose

   Goldengate/Greengate N-tag module, containing Venus fused to a flexible linker

   Depositor

   Klaus Theres

   Insert

   Venus
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGB_RT_tdTomato_li

   Plasmid

   #136964

   Purpose

   Goldengate/Greengate N-tag module, containing tdTomato fused to a flexible linker

   Depositor

   Klaus Theres

   Insert

   tandem Tomato
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGC_RT_Sa_sgRNA

   Plasmid

   #136968

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGI_RT_Sa_sgRNA

   Plasmid

   #136982

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGD_RT_Sa_sgRNA

   Plasmid

   #136975

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGD_RT_li_Tq2CFP_2NLS

   Plasmid

   #136972

   Purpose

   Goldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linker

   Depositor

   Klaus Theres

   Insert

   Tq2CFP
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGE_RT_Sa_sgRNA

   Plasmid

   #136978

   Purpose

   Goldengate/Greengate module for genome editing, using the Sa guideRNA.

   Depositor

   Klaus Theres

   Insert

   Sa guide RNA
   

   Use

   CRISPR

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-METTL14-WT

   Plasmid

   #141112

   Purpose

   Overexpression of WT METTL14 in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   HMETTL14 (METTL14 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGC_RT_XVE

    Plasmid

    #136970

    Purpose

    Goldengate/Greengate module for generation of beta-estradiole inducible genes.

    Depositor

    Klaus Theres

    Insert

    XVE
    

    Expression

    Bacterial

    Mutation

    BsaI site domestication 834 T > A,

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGA_RT_Sa_Cas9

    Plasmid

    #136962

    Purpose

    Goldengate/Greengate module for genome editing, using the SaCAS9.

    Depositor

    Klaus Theres

    Insert

    SaCAS9
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGD_RT_biChromium

    Plasmid

    #136974

    Purpose

    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide

    Depositor

    Klaus Theres

    Insert

    P2A sequence
    

    Expression

    Bacterial

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmiRA1-MYB

    Plasmid

    #141120

    Purpose

    Lentiviral overexpression of MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmiR-report-MYB-WT

    Plasmid

    #141114

    Purpose

    Reporter plasmid of WT MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    CMYB (MYB Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmiR-report-MYB-mut

    Plasmid

    #141115

    Purpose

    Reporter plasmid of mutant MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    A to T mutations at m6A sites

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCDH-METTL14-R298P

    Plasmid

    #141113

    Purpose

    Overexpression of METTL14 mutant in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    HMETTL14 (METTL14 Human)
    

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmiRA1-MYC

    Plasmid

    #141121

    Purpose

    Lentiviral overexpression of MYC in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-PIG-METTL14-WT

    Plasmid

    #141118

    Purpose

    Retroviral overexpression of WT METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-PIG-METTL14-R298P

    Plasmid

    #141119

    Purpose

    Retroviral overexpression of mutant METTL14 in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    METTL14 (METTL14 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGF_RT_FAST_Tq2CFP

    Plasmid

    #136981

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Insert

    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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