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pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Publication

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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STREP-SUMO-FLAG-SeKaiC

Plasmid

#218179

Purpose

WT SeKaiC for phosphorylation/dephosphorylation study

Depositor

Andy LiWang

Publication

Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

Insert

STREP-SUMO-FLAG-SeKaiC

Tags

STREP-Tag

Expression

Bacterial

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Publication

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-NMBR-eLOV-TEVcs-FLAG-GAL4-V5

Plasmid

#104843

Purpose

b2AR-SPARK TF component

Depositor

Alice Ting

Publication

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

NMBR-eLOV-TEVcs-FLAG-GAL4-V5

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli3/Flag

Plasmid

#51246

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template

Plasmid

#235608

Purpose

Repair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPR

Depositor

Maria Barna

Publication

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Rpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms

Use

CRISPR

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)

Plasmid

#221330

Purpose

Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

FLAG-IRSp53 and HA-Rac1(G12V) (Baiap2, Rac1 Mouse)

Use

Retroviral

Mutation

G12V

Promoter

TRE3G

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (GD)

Plasmid

#112772

Purpose

express Flag-RagC (G domain only)

Depositor

Roberto Zoncu

Publication

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Publication

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3.1-Flag-H2A K118-119R

Plasmid

#63564

Purpose

Expression of human H2A with K118R and K119R tagged with Flag

Depositor

Titia Sixma

Publication

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 118 and Lysine 119 are now Arginine

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMH-Flag-HA

Plasmid

#101766

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion protein

Depositor

Michael Huen

Publication

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Flag-HA

Expression

Mammalian

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRK5-FLAG-UFM1

Plasmid

#134640

Purpose

Expression of FLAG-tagged human UFM1 wildtype

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Publication

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Tags

6xHIS and Flag

Expression

Insect

Promoter

OpIE2

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Publication

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCDH-3xFLAG-GFP-puroR

Plasmid

#167463

Purpose

3rd-gen lentiviral expression of FLAG-GFP in mammalian cells

Depositor

Steven Artandi

Publication

Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEGFP-FLAG-AKAP-Lbc

Plasmid

#67571

Purpose

Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

Depositor

Graeme Carnegie

Publication

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

A-kinase anchoring protein Lbc (AKAP13 Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

R2604G in AKAP

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcsII-pb5-flag

Plasmid

#86769

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 5 (PSMB5 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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