161,398 results

1. pYPQ202

   Plasmid

   #86198

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis ubiquitin 10 (AtUbi10)

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEFIRES-P-EGFP-ADRP

   Plasmid

   #87157

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   mCherry-R TTGGTCACCTTCAGCTTGG

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. G5-CD40

   Plasmid

   #121166

   Purpose

   pMP71 vector expressing GFP - P2A - G5 - myc - CD40

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   G5-CD40
   

   Use

   Retroviral

   Tags

   GFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHM6-alphasynuclein-A53T

   Plasmid

   #40825

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   6x His and HA

   Expression

   Mammalian

   Mutation

   A53T

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1

   Plasmid

   #69496

   Purpose

   pJL1 expression vector with sfGFP

   Depositor

   Michael Jewett

   Article

   Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

   Insert

   Super folder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSEss-CHIg-mG1_M18

   Plasmid

   #82357

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-CAAX

   Plasmid

   #86056

   Purpose

   A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Homo sapiens Harvey rat sarcoma (HRAS Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains the region from 825-857 of HRAS as a far…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA-dCas9-FLp300

   Plasmid

   #61356

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. BL21 ΔrecBCD

    Bacterial Strain

    #176581

    Purpose

    E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression.

    Depositor

    Chase Beisel, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Bacterial Resistance

    None

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only