Addgene

We narrowed to 1,720 results for: ncl

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pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

Plasmid

#185061

Purpose

Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

Depositor

Bobby Koeleman

Insert

U6:pegRNA:scaffold:PBS+RT template

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSC301b

Plasmid

#31852

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (including ATG start codon)

Use

Tags

Expression

Bacterial

Mutation

Promoter

SOD and none

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
p203 Dync1li1 N235Y

Plasmid

#26451

Depositor

Elizabeth Fisher

Insert

cytoplasmic dynein light intermediate chain 1 N235Y (Dync1li1 Mouse)

Use

Subcloning and sequencing

Tags

Expression

Mutation

changed Asparagine 235 to Tyrosine

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only
pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGFAP::MyrGFP_v2

Plasmid

#160277

Purpose

Expresses GFP from the Gfa2 promoter. This plasmid is a new version of Plasmid 22672 and includes a stop codon in the myrEGFP insert.

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

myrGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Gfa2

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRS426GFP-2×PH(PLCδ)

Plasmid

#36092

Depositor

Scott Emr

Article

Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

Insert

2xPH(PLCdelta) fusion

Use

Tags

GFP

Expression

Yeast

Mutation

contains aa11-140 of PLCδ (including the PH domai…

Promoter

PRC1

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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pTSA-C

Plasmid

#17329

Depositor

Takeshi Sano

Article

Reznik et al Bioconjug Chem. . 12(6):1000-4.

Insert

streptavidin

Use

Tags

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Promoter

Available since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pYS104

Plasmid

#1190

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

Hsp104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type Hsp104 sequence with 588bp of 5' p…

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
pIA035

Plasmid

#200426

Purpose

amyE::(Ppen-lacIΔ11-dronPA cm). PALM integration vector includes dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

dronPA

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIA027

Plasmid

#200418

Purpose

amyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Type

Empty backbone

Use

Tags

dronPA

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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