We narrowed to 3,335 results for: UBC

1. pEGFP-C1-IFT38

   Plasmid

   #218723

   Purpose

   Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT38 (CLUAP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-FKBP-E2

   Plasmid

   #175243

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETDUET-GST AO7RE Y165A

   Plasmid

   #139428

   Purpose

   GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed tyrosine 165 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETDUET-GST AO7RE P199A

   Plasmid

   #139427

   Purpose

   GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed proline 199 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETDUET-GST AO7RE I137A

   Plasmid

   #139319

   Purpose

   GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Changed isoleucine 137 to an alanine

   Promoter

   T7 promoter-1

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MAC_N_TBG1

   Plasmid

   #111696

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TBG1 (TUBG1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MAC_C_COX14

   Plasmid

   #111663

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   COX14 (C12orf62 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MAC_C_AKIP

   Plasmid

   #111660

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   AKIP (AURKAIP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAC_C_MGST3

   Plasmid

   #111670

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   MGST3 (MGST3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-SUMO1

    Plasmid

    #185637

    Purpose

    expression of GST-tagged SUMO1 in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    small ubiquitin-like modifier 1 (SUMO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

    Plasmid

    #133027

    Purpose

    Expresses EGFP-tagged late endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB7A (RAB7A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human elongation factor 1 alpha

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAC_N_MED13

    Plasmid

    #111689

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    MED13 (MED13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

    Plasmid

    #216125

    Purpose

    Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

    Plasmid

    #134868

    Purpose

    Expresses EGFP-tagged lysosomal marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV_Thy1StTA

    Plasmid

    #97411

    Purpose

    TET driver for amplified expression in cortical neurons

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse thy1PSs

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)mGAT1YFP28

    Plasmid

    #41673

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 28 C-terminal-…

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1a-CCO-IRES-Puromycin

    Plasmid

    #134861

    Purpose

    Expresses EGFP-tagged inner mitochondrial membrane marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    cytochrome c oxidase subunit VIII (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid

    #216124

    Purpose

    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge