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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,082 results for: VARS

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Showing: 1441 - 1460 of 12082 results

  1. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

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  4. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid
    #218863
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8m
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    A25G F286Y
    Promoter
    hsp70
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218863-plasmid-map-sequence-id-434232

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  11. SiriusGFP

    Plasmid
    #123201
    Purpose
    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.
    Depositor
    Joseph Santos-Sacchi
    Article
    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76
    Insert
    SiriusGFP
    Use
    Mouse Targeting
    Tags
    N/A
    Expression
    Bacterial and Mammalian
    Mutation
    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    123201-plasmid-map-sequence-id-239192

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  12. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid
    #164193
    Purpose
    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)
    Depositor
    Thomas Oertner
    Article
    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820
    Inserts
    CamPARI2
    Human α Tubulin
    Use
    AAV
    Tags
    6 His tag and NES
    Expression
    Mammalian
    Mutation
    F391W-L398V
    Promoter
    Syn
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    164193-plasmid-map-sequence-id-325364

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Showing: 1441 - 1460 of 12082 results