We narrowed to 11,622 results for: Vars

1. pIRES2_EGFP_KCNQ1

   Plasmid

   #173161

   Purpose

   For mammalian expression of KCNQ1 and creating variants

   Depositor

   Al George

   Article

   Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

   Insert

   KCNQ1 (KCNQ1 Human)
   

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM33_AP2B1_adaptin

   Plasmid

   #178467

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   AP2B1_adaptin (AP2B1 Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-Frb:Akt2-IRES-Hygromycin

   Plasmid

   #120713

   Purpose

   Expresses Frb:Akt2 fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   AKT2 (AKT2 Human)
   

   Use

   Lentiviral

   Tags

   FRB(T2098L)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218863

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM41_KPNB1_HEAT

   Plasmid

   #178494

   Purpose

   Bacterial expression of human domain with His-tag and MBP tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KPNB1_HEAT (KPNB1 Synthetic, Human)
   

   Tags

   His-MBP

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS SARS-CoV-2 XBB Spike

   Plasmid

   #195287

   Purpose

   Expresses codon-optimized full length SARS-CoV-2 XBB Spike

   Depositor

   Marceline Côté

   Insert

   SARS-CoV-2 XBB Spike (S )
   

   Expression

   Mammalian

   Mutation

   Contains the following mutations: T19I, LPPA24-27…

   Promoter

   chicken β-actin promoter, CMV enhancer

   Available Since

   Feb. 8, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pETM33_CLIP_CAP_Gly

   Plasmid

   #178470

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CLIP_CAP_Gly (CLIP1 Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

    Plasmid

    #164193

    Purpose

    Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    F391W-L398V

    Promoter

    Syn

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM41_KPNA4_Arm

    Plasmid

    #178478

    Purpose

    Bacterial expression of human domain with His-tag and MBP tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KPNA4_Arm (KPNA4 Synthetic, Human)
    

    Tags

    His-MBP

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM33_MDM2_SWIB

    Plasmid

    #178481

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MDM2_SWIB (MDM2 Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ETS1_pet28a

    Plasmid

    #131663

    Purpose

    Bacterial expression of ETS gene ETS1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETS1 (ETS1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MAC_N_KLF6

    Plasmid

    #167772

    Purpose

    Expresses KLF6 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    KLF6 (KLF6 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM33_EIF4E_eiF4E

    Plasmid

    #178474

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    EIF4E_eiF4E (EIF4E Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM33_USP7_MATH

    Plasmid

    #178490

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    USP7_MATH (USP7 Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-FKBP-GFP-NDP52

    Plasmid

    #135296

    Purpose

    stably express GFP-tagged NDP52 for CID induced mitophagy

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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