Addgene

We narrowed to 1,475 results for: asl;

Showing: 1441 - 1460 of 1475 results

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UAS-u(XXL)Cas9

Plasmid

#127387

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XXL)-Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCKIPSD-1/1

Plasmid

#91376

Purpose

Protein expression and purification of human SH3 domain construct NCKIPSD-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCKIPSD-1/1 (NCKIPSD Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc

Plasmid

#198113

Purpose

Express hCOL4A5 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_R1683X

Plasmid

#198124

Purpose

Express hCOL4A5_R1683X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

R1683X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Use

Tags

3x HA, V5

Expression

Insect

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 Δ593-618

Plasmid

#47901

Purpose

Expresses myc tagged Miro2 lacking transmembrane domain

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 Δ593-618 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Δ593-618, lacks transmembrane domain. Also see De…

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Tlr4(Lps)

Plasmid

#27149

Depositor

Bruce Beutler

Article

Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

Insert

toll-like receptor 4(Lps) (Tlr4 Mouse)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed Proline 712 to Histidine. Native signal p…

Promoter

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1632X

Plasmid

#198122

Purpose

Express hCOL4A5_S1632X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

S1632X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_Q1016X

Plasmid

#198119

Purpose

Express hCOL4A5_Q1016X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

Q1016X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_G5X

Plasmid

#198114

Purpose

Express hCOL4A5_G5X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

G5X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_C29X

Plasmid

#198115

Purpose

Express hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

C29X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_E130X

Plasmid

#198117

Purpose

Express hCOL4A5_E130X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

E130X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S36X

Plasmid

#198116

Purpose

Express hCOL4A5_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

S36X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_S1661X

Plasmid

#198123

Purpose

Express hCOL4A5_S1661X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Tags

Nluc

Expression

Mammalian

Mutation

S1661X

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1 (BTLA ITIM)-mGFP

Plasmid

#180817

Purpose

Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

PD-1 (BTLA ITIM) (PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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