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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,439 results for: golden gate

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Showing: 1441 - 1460 of 5439 results

  3. pGA-red-mini

    Plasmid
    #196338
    Purpose
    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
    Depositor
    Paola Branduardi
    Article
    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly backbone
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196338-plasmid-map-sequence-id-393164

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  4. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

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  5. pENTR_AtU6p_BsaI_gRNA

    Plasmid
    #225987
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225987-plasmid-map-sequence-id-448010

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  6. pPD765 TRE3GV MCS in TU1

    Plasmid
    #139253
    Purpose
    TRE3GV promoter and an MCS in TUPV1
    Depositor
    Joshua Leonard
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139253-plasmid-map-sequence-id-325169

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  7. pACT-HAB1-backbone

    Plasmid
    #241287
    Purpose
    Backbone for HAB1 mutants library construction from oligopools; PYR1 sensor library assembly from oligopools;
    Depositor
    Sean Cutler
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    241287-plasmid-map-sequence-id-484463

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  8. pCENTR_DMC1p_amiRNA

    Plasmid
    #225985
    Purpose
    To drive any artificial microRNA under the regulatory control of AtDMC1 promoter and it will make a gateway entry clone
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225985-plasmid-map-sequence-id-448024

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  9. pPD767 TRE3GV MCS in TU4

    Plasmid
    #139256
    Purpose
    TRE3GV promoter and an MCS in TUPV4
    Depositor
    Joshua Leonard
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139256-plasmid-map-sequence-id-325173

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  10. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

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  11. pRDA_887

    Plasmid
    #245329
    Purpose
    Cas12a CRISPRa cloning vector; contains NbALFA, p65
    Depositor
    John Doench, David Root
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245329-plasmid-map-sequence-id-488027

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  12. pJJB1434

    Plasmid
    #239494
    Purpose
    Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome
    Depositor
    John Dueber
    Insert
    ScErg20p(F96W_N127W)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239494-plasmid-map-sequence-id-475871

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  13. pKS1024

    Plasmid
    #239495
    Purpose
    Express peroxisomal RsAS
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS-Venus-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239495-plasmid-map-sequence-id-475870

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  14. pBD016

    Plasmid
    #201307
    Purpose
    Sr27 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    Sr27
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    201307-plasmid-map-sequence-id-410032

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  15. pPD1159 pLink 9

    Plasmid
    #139247
    Purpose
    Vector that holds the linker for circuits with 9 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker9
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139247-plasmid-map-sequence-id-325057

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  16. pPD1158 pLink 8

    Plasmid
    #139246
    Purpose
    Vector that holds the linker for circuits with 8 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker8
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139246-plasmid-map-sequence-id-325056

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  17. pPD1157 pLink 7

    Plasmid
    #139245
    Purpose
    Vector that holds the linker for circuits with 7 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker7
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139245-plasmid-map-sequence-id-325055

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  18. pPD1156 pLink 6

    Plasmid
    #139244
    Purpose
    Vector that holds the linker for circuits with 6 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker6
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139244-plasmid-map-sequence-id-325054

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  19. pPD1155 pLink 5

    Plasmid
    #139243
    Purpose
    Vector that holds the linker for circuits with 5 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker5
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139243-plasmid-map-sequence-id-325051

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  20. pPD1154 pLink 4

    Plasmid
    #139242
    Purpose
    Vector that holds the linker for circuits with 4 TUs
    Depositor
    Joshua Leonard
    Insert
    Linker4
    Use
    Synthetic Biology
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    139242-plasmid-map-sequence-id-325049

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Showing: 1441 - 1460 of 5439 results