We narrowed to 3,816 results for: golden gate

1. pLII-proUBI10_CKA3 KD K63M,N170D-FLAG_termNOS _OLE-RED

   Plasmid

   #238523

   Purpose

   Plant expression of flag-tagged A. thaliana CK2alpha3 KD K63M,N170D; with OLE1-RED marker

   Depositor

   Michael Hothorn

   Insert

   AtCK2alpha3 (AT2G23080 Mustard Weed)
   

   Tags

   FLAG

   Expression

   Plant

   Mutation

   K63M N170D

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG1016-2xBsaI-SaKKH_P2A_EGFP

   Plasmid

   #239461

   Purpose

   Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.

   Depositor

   Alexis Komor

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MERS-CoV Mpro strain HCoV-EMC 3CL protease domain

   Plasmid

   #228646

   Purpose

   Bacterial expression of the MERS-CoV Mpro domain for biochemical assay.

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   MERS-Cov Mpro domain
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   March 28, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAIP4_1T3Y

   Plasmid

   #234228

   Purpose

   Heterologous protein expression of coactosin-like protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1T3Y (COTL1 Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAIP4_1JL1

   Plasmid

   #234206

   Purpose

   Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1JL1 (rnhA Escherichia coli)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAIP4_3TOW

   Plasmid

   #234201

   Purpose

   Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3TOW (MVB12B Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAIP4_1KQX

   Plasmid

   #234181

   Purpose

   Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1KQX (rbp2a Zebrafish)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAIP4_1AKO

   Plasmid

   #234162

   Purpose

   Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1AKO (xthA Escherichia coli K-12)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAIP4_4NFB

   Plasmid

   #234161

   Purpose

   Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4NFB (PILRA Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease

    Plasmid

    #228638

    Purpose

    Bacterial expression of the Coxsackievirus 63 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Coxsackievirus 63 3C protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219690

    Purpose

    Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid

    #219688

    Purpose

    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2BP/p35S (BFP, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid

    #219686

    Purpose

    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

    Depositor

    Jennifer Nemhauser

    Insert

    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    

    Expression

    Plant

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MUT_S7-H1

    Plasmid

    #218396

    Purpose

    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    PPhlF

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. IBMc050

    Plasmid

    #161938

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    

    Expression

    Bacterial

    Promoter

    Plux2

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. IBMc318

    Plasmid

    #161941

    Purpose

    Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-G-ER-LBD-S-SapI
    

    Expression

    Bacterial

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGImC_V5-CSNK1A1

    Plasmid

    #92014

    Purpose

    Expresses V5-CSNK1A1, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGM4723:Ble:Cas9

    Plasmid

    #112208

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

    Depositor

    Thomas Mock

    Inserts

    Cas9
    SheBle
    

    Use

    CRISPR; Diatom, phaeodactylum

    Tags

    Phaeodactylum fcp promoter and terminator and YFP

    Mutation

    Phaeodactylum Fcp promoter

    Promoter

    Phaeodactylum Fcp

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus A71 3C protease isoform B
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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