We narrowed to 3,026 results for: human cdnas

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K592A

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-NSF/T646A

Plasmid

#74937

Purpose

Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

Depositor

Elisa Greggio

Article

Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

Insert

NSF (NSF Human)

Tags

FLAG

Expression

Mammalian

Mutation

T646A (mutation in the D2 domain of the protein)

Promoter

CMV

Available Since

April 26, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

Plasmid

#205603

Purpose

Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinWT-RNAi-resistant-GFP

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CIM5 (Rpt-1) GFP

Plasmid

#16074

Depositor

Erin Schuman

Article

Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

Insert

CIM5 (RPT1 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_V4146I-eGFP

Plasmid

#108405

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4137P-eGFP

Plasmid

#108403

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Tags

3xHA and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-HsPcdh21-Myc-Flag-His

Plasmid

#210818

Purpose

Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3x Flag, 10x His

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArA K162R

Plasmid

#59805

Purpose

Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora A (AURKA Human)

Tags

Myc

Expression

Mammalian

Mutation

K162R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso2 S172A

Plasmid

#74392

Purpose

Flag tagged human S172A MFF isoform 2 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO Myc ArB WT RNAiR

Plasmid

#59806

Purpose

Allows the integration of Myc ArB in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

RNAi resistant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC Long WT

Plasmid

#59809

Purpose

Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso1 S172A

Plasmid

#74391

Purpose

Flag tagged human S172A MFF isoform 1 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26) and Ser 172 Ala.

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5 TO FRT Myc ArB K106R

Plasmid

#59807

Purpose

Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

K106R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC K72R

Plasmid

#59811

Purpose

Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Mutation

K72R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso4 S172A

Plasmid

#74393

Purpose

Flag tagged human S172A MFF isoform 4 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 myc-Rab10-mito

Plasmid

#208367

Purpose

Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

myc-Rab10-mito (RAB10 Human)

Expression

Mammalian

Mutation

wildtype

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(Hygro) myc-hPolQ-Flag

Plasmid

#73132

Purpose

To express in mammalian cells human PolQ

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

Polymerase theta (POLQ Human)

Tags

Flag and myc

Expression

Mammalian

Promoter

cmv promoter

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase S1226A/ C2029A

Plasmid

#59823

Purpose

Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

S1226A/ C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

Plasmid

#205604

Purpose

Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

Depositor

Tarun Kapoor

Article

Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

Insert

γ-tubulinΔGTP-RNAi-resistant-GFP

Tags

GFP

Expression

Mammalian

Mutation

G2228A, C2231T, C2237T, G2240A, N229A

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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