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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,133 results for: puc

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Showing: 3651 - 3700 of 5133 results

  10. pMDW108

    Plasmid
    #227194
    Purpose
    Template for multi-nucleosome array
    Depositor
    Michelle Wang
    Article
    Le et al Cell. 2019 Oct 17;179(3):619-631.e15. do
    Insert
    64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
    Expression
    Bacterial
    Promoter
    Plac
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227194-plasmid-map-sequence-id-461742

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  13. pHBJT002

    Plasmid
    #225153
    Purpose
    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225153-plasmid-map-sequence-id-443288

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  28. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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  29. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

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  30. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Mutation
    N/A (synthetic)
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

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  32. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Expression
    Bacterial
    Promoter
    N/A
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  46. pTS1041

    Plasmid
    #169619
    Purpose
    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169619-plasmid-map-sequence-id-366634

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  47. pVH1001-Tier2(ColE1 ori)

    Plasmid
    #169599
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169599-plasmid-map-sequence-id-366207

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  49. pTS1040

    Plasmid
    #169618
    Purpose
    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169618-plasmid-map-sequence-id-365617

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Showing: 3651 - 3700 of 5133 results