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Showing: 3651 - 3700 of 4956 results

  4. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK1
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

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  5. AICSDP-27:SLC25A17-mEGFP

    Plasmid
    #101784
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17
    Depositor
    Allen Institute for Cell Science
    Insert
    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101784-plasmid-map-sequence-id-197677

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  8. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid
    #101785
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Insert
    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101785-plasmid-map-sequence-id-197676

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  9. AICSDP-41: ATP2A2-mEGFP

    Plasmid
    #114405
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2
    Depositor
    Allen Institute for Cell Science
    Insert
    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114405-plasmid-map-sequence-id-220830

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  11. pMT950

    Plasmid
    #228290
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    EGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228290-plasmid-map-sequence-id-457112

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  12. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175508
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175508-plasmid-map-sequence-id-345097

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  15. pDGE334

    Plasmid
    #153243
    Purpose
    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153243-plasmid-map-sequence-id-305787

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  16. pDGE337

    Plasmid
    #153246
    Purpose
    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153246-plasmid-map-sequence-id-305804

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  17. qTAG-AAVS1-Ef1a-Puro

    Plasmid
    #227266
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227266-plasmid-map-sequence-id-452014

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  19. AICSDP-50: NPM1-mEGFP

    Plasmid
    #109122
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109122-plasmid-map-sequence-id-209780

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  24. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Article
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    118757-plasmid-map-sequence-id-231795

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  27. TC1316

    Plasmid
    #164883
    Purpose
    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3
    Depositor
    Tian Chi
    Article
    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:
    Insert
    dcas13d-ADARdd
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dCas13d L560-G586 deleted
    Promoter
    CMV, T7
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164883-plasmid-map-sequence-id-325957

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  28. AICSDP-72:TFAM-mEGFP

    Plasmid
    #159745
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM
    Depositor
    Allen Institute for Cell Science
    Insert
    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159745-plasmid-map-sequence-id-314375

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  31. pDGE476

    Plasmid
    #153267
    Purpose
    M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153267-plasmid-map-sequence-id-307092

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  34. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  36. qTAG-AAVS1-PGK-Puro

    Plasmid
    #227269
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227269-plasmid-map-sequence-id-452020

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  37. qTAG-AAVS1-Ef1a-Zeo

    Plasmid
    #227268
    Purpose
    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227268-plasmid-map-sequence-id-451274

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  39. pCE38

    Plasmid
    #174432
    Purpose
    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174432-plasmid-map-sequence-id-349725

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  40. pCE41

    Plasmid
    #174433
    Purpose
    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174433-plasmid-map-sequence-id-349717

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  41. pCE27

    Plasmid
    #174405
    Purpose
    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174405-plasmid-map-sequence-id-349710

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  43. pCE35

    Plasmid
    #174409
    Purpose
    C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174409-plasmid-map-sequence-id-349711

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  50. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid
    #175507
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175507-plasmid-map-sequence-id-345876

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Showing: 3651 - 3700 of 4956 results