We narrowed to 150,436 results for: ins

1. pST_APOA4_ANDDA_52-358 (pBS0744)

   Plasmid

   #185235

   Purpose

   For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_52-358. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   APOA4_ANDDA_52-358
   

   Expression

   Mammalian

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnEA-vH_His-TEV-SEPT2

   Plasmid

   #174491

   Purpose

   bacterial expression of human SEPT2

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CAG-ChR2-YFP

   Plasmid

   #204345

   Purpose

   PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   ChR2-YFP
   

   Tags

   Yellow fluorescent protein

   Expression

   Mammalian

   Mutation

   C-terminus (aa315-737) is deleted - PMID: 1461559…

   Promoter

   CAG

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShuttle CMV RGECO-TnT

   Plasmid

   #124643

   Purpose

   Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

   Depositor

   Hugh Watkins, Matthew Daniels, Paul Robinson

   Article

   Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

   Insert

   RGECO-TnT (TNNT2 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pShuttle CMV RGECO-TnI

   Plasmid

   #124642

   Purpose

   Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination

   Depositor

   Hugh Watkins, Matthew Daniels, Paul Robinson

   Article

   Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

   Insert

   RGECO-TnI (TNNI3 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. UCP3 promoter EP1

   Plasmid

   #71743

   Purpose

   Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity

   Depositor

   Heinrich Taegtmeyer

   Article

   Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.

   Insert

   Uncoupling Protein 3 promoter (Ucp3 Mouse)
   

   Tags

   Luciferase

   Expression

   Bacterial

   Mutation

   Contains 7kb of the murine UCP3 gene encompassing…

   Promoter

   UCP3

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGS-CD64-2

   Plasmid

   #109191

   Purpose

   Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   Full-length engineered CD64, derived from human CD64
   

   Tags

   Twin-Strep

   Expression

   Insect

   Mutation

   engineered for efficient expression, see publicat…

   Promoter

   Polyhedrin

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCold I-Hero13

    Plasmid

    #187929

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero13

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero13 (BEX3 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

    Plasmid

    #185309

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnCS_SEPT6_SEPT7-TEV-Strep

    Plasmid

    #174499

    Purpose

    bacterial co-expression of human SEPT6 and of human SEPT7

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN6 (SEPTIN6 Human)
    SEPTIN7 (SEPTIN7 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-vH_His-TEV-SEPT2-msfGFP

    Plasmid

    #174492

    Purpose

    bacterial expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCold I-Hero20

    Plasmid

    #187930

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero20

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero20 (C11orf58 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBa-LSS-GFP-LDLR wt

    Plasmid

    #98184

    Purpose

    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

    Insert

    LDLR (LDLR Human)
    

    Tags

    GFP and LDLR signal sequence, N-term of GFP so GF…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta Actin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnCS_SEPT7_SEPT9_i5-TEV-Strep

    Plasmid

    #174502

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i5

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-N-Myc_535-1102 USP7

    Plasmid

    #131246

    Purpose

    Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Expresses USP7 amino acids 535-1102. This truncat…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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