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We narrowed to 4,550 results for: pCMV mammalian

Showing: 1461 - 1480 of 4550 results

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA + S580A POLI

Plasmid

#131241

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert Pol iota se…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

Plasmid

#163554

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-NG-nCas9-RBMX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

Plasmid

#163552

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-nCas9-RBMX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Plasmid

#163545

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Use

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV_iPE-C_P2A_Csy4

Plasmid

#232428

Purpose

Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

Insert

iPE-C–P2A–Csy4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-N-HA-Gp78

Plasmid

#123591

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV p16 INK4A

Plasmid

#10916

Depositor

Bob Weinberg

Article

Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

Insert

p16 INK4 (CDKN2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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p3258 pCMV hUBC9 wt HA

Plasmid

#14438

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 (UBE2I Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Article

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Insert

SMAD3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Sep 9. ():.

Insert

PTEN (PTEN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock

Plasmid

#47334

Purpose

full length clock in pCMV10 with 3x flag at the N-terminus

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

clock

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Use

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC2

Plasmid

#173468

Purpose

Human PGRMC2 containing an N-terminal FLAG tag

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-VSV-G-Myc

Plasmid

#80054

Purpose

Expression of a Myc tagged VSV-G protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VSV-G-Myc

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B-NCreERT2

Plasmid

#106370

Purpose

N-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

NCreERT2

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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