We narrowed to 13,833 results for: cas9

1. pX459-dTLN1 intron

   Plasmid

   #176224

   Purpose

   A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-v2-ALDH1A3gRNA2

   Plasmid

   #189747

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-v2-ALDH1A3gRNA1

   Plasmid

   #189746

   Purpose

   The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   Cas9, ALDH1A3 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168243

   Purpose

   "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-DN
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168244

   Purpose

   "Rescue Rac-CA expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-CA
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEA1-Nuclease

   Plasmid

   #176528

   Purpose

   Delivers all prime editing nuclease components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-U6-UL29-U3-UL8

   Plasmid

   #166685

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA (UL29, UL8 HSV-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

   Plasmid

   #173891

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-KalTA4; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

   Plasmid

   #160609

   Purpose

   TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2-VP64:Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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