168,238 results

1. pCMV-PE2

   Plasmid

   #132775

   Purpose

   Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   PE2
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1a-IRES-Neo

   Plasmid

   #85139

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUMVC

   Plasmid

   #8449

   Purpose

   Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MoClo Yeast Secretion and Display (YSD) Toolkit

   Plasmid Kit

   #1000000240

   Purpose

   The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.

   Depositor

   Paul Young

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

7. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSNAPf-H2B Control Plasmid

   Plasmid

   #101124

   Purpose

   Expression of SNAP-tagged Histone H2B in mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   histone cluster 1 H2B family member j (H2BC11 Human)
   

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAAV-hSynapsin1-axon-GCaMP6f (AAV1)

    Viral Prep

    #112000-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-GCaMP6f (#112000). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6f plasmid DNA. Synapsin-driven, axon-targeted GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Promoter

    Syn

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only