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MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV5B-FLAG-SARA dSBD

Plasmid

#11737

Depositor

Jeff Wrana

Article

Tsukazaki et al Cell. 1998 Dec 11. 95(6):779-91.

Insert

SARA (ZFYVE9 Human)

Tags

Flag

Expression

Mammalian

Mutation

deltaSBD: lacks Smad2-binding domain (aa665-704)

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCAGGS-AcGFP-C

Plasmid

#203325

Purpose

Express N-terminal AcGFP-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

AcGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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cavin-3-HA

Plasmid

#27711

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

cavin-3 (CAVIN3 Human)

Tags

HA

Expression

Mammalian

Mutation

R8P

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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M2660 ura3::LYS2 Disruptor Converter

Plasmid

#51678

Purpose

Yeast marker swap plasmid for converting URA3 to LYS2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-GGA(EAAAK)2AGG

Plasmid

#137104

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGA(EAAAK)2AGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Article

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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M3938 trp1::ADE2 Disruptor Converter

Plasmid

#51673

Purpose

Yeast marker swap plasmid for converting TRP1 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL.CMVenh.gp91.eGFP

Plasmid

#30468

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/EGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pC-PTP-NEO

Plasmid

#51710

Purpose

contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available Since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1 SYS1

Plasmid

#175092

Purpose

SYS1-mEGFP can be transfected in mammalian cells

Depositor

Juan Bonifacino

Article

Ishida et al J Cell Biol. 2019 Nov 4;218(11):3681-369

Insert

SYS1 (SYS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEPSW1KN0034

Plasmid

#196178

Purpose

MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

CaMV35S::TMV::LucF::flag::35sT

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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D771 leu2::LYS2 Disruptor Converter

Plasmid

#51677

Purpose

Yeast marker swap plasmid for converting LEU2 to LYS2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pIV101

Plasmid

#196671

Purpose

Enables recombination mediated gene delivery into Agrobacterium strains for subsequent transformation of plants

Depositor

Simona Radutoiu

Article

Ferguson et al PLoS One. 2023 Nov 1;18(11):e0291680. do

Type

Empty backbone

Use

Integration vector

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pICH41432

Plasmid

#50343

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH44022

Plasmid

#50330

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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