We narrowed to 4,429 results for: Erf

1. pBS NL4-3 envFS eGFP Δrev

   Plasmid

   #101348

   Purpose

   All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta rev

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB508

   Plasmid

   #74850

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-FKBP-TASL(FL)

   Plasmid

   #221263

   Purpose

   Expression of TASL in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TASL (Tasl Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-Arch-GFP

   Plasmid

   #37810

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   Arch
   

   Use

   AAV; Adeno-associated virus

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. LL - hOCT4i -2

   Plasmid

   #12197

   Depositor

   George Daley

   Article

   Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

   Insert

   OCT4 - RNAi (POU5F1 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLC-241

   Plasmid

   #73194

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-mqsR (mqsR )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWB450

   Plasmid

   #74832

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD24-rnc-sfGFP

   Plasmid

   #51560

   Purpose

   Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   rnc (rnc E. coli)
   

   Use

   Tags

   GLESTCRHASLAVLADERRFSA and superfolder GFP

   Expression

   Bacterial

   Mutation

   Contains additonal aa at the end of the sfGFP in …

   Promoter

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-FKBP-TMPRSS3

   Plasmid

   #221298

   Purpose

   Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TMPRSS3 (pLxIS) (Tmprss3 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE

    Plasmid

    #101351

    Purpose

    HIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSET a sfGCaMP6s

    Plasmid

    #100022

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfGCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCellFree_G03 IL1B

    Plasmid

    #67066

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL1B (IL1B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 CALR

    Plasmid

    #67086

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CALR (CALR Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGWB416

    Plasmid

    #74810

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB440

    Plasmid

    #74826

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Use

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRIME-CMV-LNGFR-FF3

    Plasmid

    #11666

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB523

    Plasmid

    #74865

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB507

    Plasmid

    #74849

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB423

    Plasmid

    #74817

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge