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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,905 results for: NUC

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Showing: 1481 - 1500 of 12905 results

  1. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Article
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

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  3. MLKS2delARMec

    Plasmid
    #130860
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Mutation
    Deletion of armadillo domain
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130860-plasmid-map-sequence-id-255666

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  4. MLKS2del4exp

    Plasmid
    #130861
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of terminal four amino acids
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130861-plasmid-map-sequence-id-255913

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  6. MLKS2delTMexp

    Plasmid
    #130863
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of transmembrane domain
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130863-plasmid-map-sequence-id-255916

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  8. MLKS2del4ec

    Plasmid
    #130857
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Mutation
    Deletion of terminal four amino acids
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130857-plasmid-map-sequence-id-255655

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  10. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

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  11. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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  17. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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Showing: 1481 - 1500 of 12905 results