We narrowed to 9,095 results for: Pol;

1. L2_NOP1gRNA-Cas9-CsA

   Plasmid

   #136139

   Purpose

   Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. wtVP1 only

   Plasmid

   #166673

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2267

   Plasmid

   #158392

   Purpose

   High Copy number plasmid containing Ple341 (PCP2 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple341 (PCP2 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple341

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pEMS2279

   Plasmid

   #158399

   Purpose

   High Copy number plasmid containing Ple349 (PDE6H MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple349 (PDE6H Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple349

   Available since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

   Plasmid

   #121802

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + CMV::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

   Plasmid

   #121805

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + EF1a::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KA401: pMVP (L3-L2) pA; RIP::TETa-pA

   Plasmid

   #121809

   Purpose

   pMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   polyA + RIP::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA

   Plasmid

   #121810

   Purpose

   pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag-polyA + RIP::TETa-polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRevLCav2FeS

   Plasmid

   #60808

   Purpose

   to disrupt iron sulfur cluster in yeast Rev3

   Depositor

   Youri Pavlov, Tahir Tahirov

   Article

   Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

   Insert

   REV3 (REV3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   contains 647 C-terminal amino acids and 335 bp of…

   Promoter

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCWXPGR-pTF-betaCatenin

    Plasmid

    #114281

    Purpose

    TET-inducible expression of a constitutively active form of the BetaCatenin protein

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    Catenin beta 1 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of AA 1 to 45

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    7SL upstream pollII enhancer

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

    Plasmid

    #156449

    Purpose

    For transient expression of human PDS5A tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5A (PDS5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-PARP1-3xFlag-7xKQ

    Plasmid

    #111576

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2232

    Plasmid

    #158401

    Purpose

    High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple316 (NR2E1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Ple316

    Available since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGEX4T2 RANK-L

    Plasmid

    #108571

    Purpose

    Encodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tag

    Depositor

    Jean-Claude Scimeca

    Article

    Beranger et al J Bone Miner Res. 2007 Jul;22(7):975-83.

    Insert

    RANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    aa158-aa316

    Promoter

    Ptac (trp/lac)

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

    Plasmid

    #156447

    Purpose

    For transient expression of mouse SMC3 tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SMC3 (Smc3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

    Plasmid

    #156443

    Purpose

    For transient expression of mouse BORIS (CTCFL) tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse BORIS (Ctcfl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

    Plasmid

    #156442

    Purpose

    For transient expression of mouse PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse PDS5B (Pds5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge