We narrowed to 1,545 results for: Tef;

1. pcDNA3.1-N-Myc_884-1102 USP7

   Plasmid

   #131249

   Purpose

   Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-N-Myc_775-1102 USP7

   Plasmid

   #131250

   Purpose

   Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9_sgRNA_0

   Plasmid

   #70763

   Purpose

   expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

   Inserts

   U6 promoter
   cas9
   tnos terminator
   

   Use

   Self-replicating in ustilago maydis, conferring c…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U6 from Ustilago maydis and synthetic otef promot…

   Available since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-N-Myc_DW164AA USP7

   Plasmid

   #131252

   Purpose

   Mammalian expression of N-terminally Myc-tagged DW164AA USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 aspa…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastbac1-GST-hUSP7

   Plasmid

   #63573

   Purpose

   Expression of synthetic human USP7 tagged with GST

   Depositor

   Titia Sixma

   Article

   Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

   Insert

   USP7 (USP7 Synthetic, Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Synthetic based on human protein sequence (please…

   Promoter

   Polyhedrin

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_Tau Authentic Alu 10-12V337M

   Plasmid

   #194169

   Purpose

   Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 V337M
   

   Use

   Tags

   3X flag

   Expression

   Mammalian

   Mutation

   Changed Valine 337 to Methinonine

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_SNORD27h_2ntmut

   Plasmid

   #73067

   Purpose

   expression clone for human mutated SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (E2F7 Human)
   

   Use

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   2 nt mutation in AS box that binds E2F7 pre-mRNA

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1_hE2F7mg_PP7-tag

   Plasmid

   #73078

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   5'-PP7-tag RNA

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_hE2F7mg_2ntmut

   Plasmid

   #73074

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns, 2…

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

10. WT USP7 UBL-12

    Plasmid

    #227230

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGS539_rPD-L1nb

    Plasmid

    #226717

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Recombinant PD-L1 nanobody
    

    Use

    Tags

    HA tag, Histidine tag, and Mating factor alpha se…

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOGS539_GLuc

    Plasmid

    #226718

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_Tau Authentic Alu 10-12K317M

    Plasmid

    #194168

    Purpose

    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 K317M
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Lysine 317 to Methionine

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_hE2F7mg_6ntmut

    Plasmid

    #73075

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1_hE2F7mg_10ntmut

    Plasmid

    #73076

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns wi…

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_SPOP_WT

    Plasmid

    #81856

    Purpose

    Gateway Donor vector containing SPOP ,part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_SPOP_p.F133S

    Plasmid

    #81637

    Purpose

    Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F133S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223_SPOP_p.W131G

    Plasmid

    #81632

    Purpose

    Gateway Donor vector containing SPOP , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    W131G

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_SPOP_p.F102C

    Plasmid

    #81642

    Purpose

    Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPOP (SPOP Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F102C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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