We narrowed to 6,901 results for: plasmid dna

1. pLV-SFFV-MCS-WPRE-UbC-mCherry

   Plasmid

   #211799

   Purpose

   Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   multiple cloning site
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SFFV-mCherry-WPRE-UbC-MCS

   Plasmid

   #211801

   Purpose

   Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   mCherry
   multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. BL21 Rosetta2 ΔrecBCD GamS

   Bacterial Strain

   #176586

   Purpose

   E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol and ampicillin

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

4. BL21 Rosetta2 ΔrecB GamS

   Bacterial Strain

   #176585

   Purpose

   E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol and ampicillin

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pLV-SFFV-MCS-WPRE-UbC-MCS

   Plasmid

   #211798

   Purpose

   Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   multiple cloning site
   multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-SFFV-MCS-WPRE-UbC-Emerald

   Plasmid

   #211796

   Purpose

   Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   multiple cloning site
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-SFFV-Emerald-WPRE-UbC-MCS

   Plasmid

   #211800

   Purpose

   Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   Emerald
   multiple cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPI

   Plasmid

   #12254

   Purpose

   2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EMCV IRES-EGFP cassette

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC119

   Plasmid

   #50010

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Vieira et al Methods Enzymol. 1987;153:3-11.

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC118

    Plasmid

    #50009

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Vieira et al Methods Enzymol. 1987;153:3-11.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xAP-1_RE::Renilla

    Plasmid

    #178317

    Purpose

    AP-1 Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    AP-1 Renilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7xSMAD_RE::GrRenilla

    Plasmid

    #178319

    Purpose

    SMAD GreenRenilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    SMAD GreenRenilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVdual-CMV-eGFP

    Plasmid

    #230931

    Purpose

    pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAIP4_1AKZ

    Plasmid

    #234172

    Purpose

    Heterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1AKZ (UNG Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOpen-RNAse A

    Plasmid

    #165565

    Purpose

    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    RNAse A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLSLGFP

    Plasmid

    #51494

    Purpose

    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GFP
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLSLT

    Plasmid

    #51498

    Purpose

    pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    T30 TALEN pair
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AH6112

    Bacterial Strain

    #166036

    Purpose

    Loopometer recipient strain: lacI- Oid+ control

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Bacterial Resistance

    Ampicillin and spectinomycin

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only