164,599 results

1. pEGFP-N3-FLuc-7BoxB_O

   Plasmid

   #147080

   Purpose

   Mammalian Expression of 7BoxB

   Depositor

   Elisa Izaurralde

   Insert

   7BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-ND-Cdt1ΔR198A/R210A-HA-Puro

   Plasmid

   #193769

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1ΔR198A/R210A (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-ND-Cdt1Δ499-546-HA-Puro

   Plasmid

   #193768

   Purpose

   Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA ) w/ mutations stopping S phase degradation and in MCM binding domain (Δ499-546), expressed from all-in-one pCW vector (TetOn + rtTA)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   ND-Cdt1Δ499-546 (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

   Promoter

   TRE

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF0234

   Plasmid

   #142487

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MNX1 (MNX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWT35S-TIM21(1–50)-Citrine

   Plasmid

   #182584

   Purpose

   Transient expression of TIM21(1–50)-Citrine in plant cell (Mitochondria)

   Depositor

   Yutaka Kodama

   Article

   Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

   Insert

   TIM21(1–50)
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N3-FLuc-5BoxB_O

   Plasmid

   #147079

   Purpose

   Mammalian Expression of 5BoxB

   Depositor

   Elisa Izaurralde

   Insert

   5BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TPC2(E643A)-mCherry

   Plasmid

   #135187

   Purpose

   Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Mutation of glutamate 643 to alanine

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-191084-gp160

   Plasmid

   #123234

   Purpose

   Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (191084 B7-19) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IL1RN gRNA4

   Plasmid

   #113133

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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