We narrowed to 11,502 results for: tet on
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Plasmid#157985PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN Incenp
Plasmid#108481Purposeinducible expression of vsv-CenpB-1-166-dCEN-INCENPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
TagsVSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121800PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP
Plasmid#108485Purposeinducible expression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
TagsEGFP and VSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)
Plasmid#70740Purposeplasmid for building 3n2, 5n1, 5n2DepositorInsertphlF-ssrA
Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)
Plasmid#70736Purposeplasmid for building 3n2DepositorInserttetR-ssrA
Available SinceFeb. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-VP64-MCS_N-term
Plasmid#136886PurposeDoxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertVP64 activator domain
UseLentiviralTagsFlagExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-KRAB-MCS_N-term
Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)
Plasmid#160570PurposeA version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffoldDepositorInsertsgRNA:scaffold tetraloop MS2 aptamer SAM
UseCRISPRMutationBsaI and BsmBI sites removedAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121804PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121808PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L900A(pL91)
Plasmid#124395PurposeE.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L900A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationL900AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898E(pL91)
Plasmid#124394PurposeE.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898E (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationT898EAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898A(pL91)
Plasmid#124393PurposeE.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationT898AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L897A(pL91)
Plasmid#124392PurposeE.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L897A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationL897AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-I894A(pL91)
Plasmid#124391PurposeE.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-I894A (TET1 Human)
TagsGB1-HistidineExpressionBacterialMutationI894AAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)
Plasmid#91874PurposeRapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2DepositorInsertRapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
TagsmCherryExpressionBacterial and MammalianAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)
Plasmid#70733Purposeplasmid for building 5n1DepositorInserttetR-ssrA
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)
Plasmid#70739Purposeplasmid for building 5n2DepositorInserttetR-ssrA
Available SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only