We narrowed to 11,717 results for: 158
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Plasmid#190573PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only
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plx307 SHOC2 V5 M173I
Plasmid#190574PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 M173V
Plasmid#190575PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 R223F
Plasmid#190576PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 T411A
Plasmid#190577PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 V64G
Plasmid#190578PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2V5 N434D
Plasmid#190579PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 R182E
Plasmid#190580PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
plx307 SHOC2 V5 M219V
Plasmid#190581PurposeSHOC2 variant overexpressionDepositorAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF645
Plasmid#88318PurposeDonor Vector containing ZNF645 transcription factor, part of the Human TFome CollectionDepositorInsertZNF645 (ZNF645 Human)
UseGateway CloningMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R
Plasmid#166814PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E
Plasmid#166811PurposeMammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contactsDepositorInsertNLRP1 (NLRP1 Human)
TagsFLAGExpressionMammalianMutationLL1193EE, P1214R, K1277EPromoterCMVAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G
Plasmid#166812PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E
Plasmid#166813PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S RK96EE
Plasmid#166797PurposeMammalian cell expression vector for FLAG-tagged DPP9S. RK96EE mutant is partial loss of CARD8 binding.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL102EE
Plasmid#166799PurposeMammalian cell expression vector for FLAG-tagged DPP9S. LL102EE mutant is partial loss of CARD8 binding.DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA2
Plasmid#131140PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-sim1
Plasmid#67908PurposeInducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.DepositorInsertPIAL2 (AT5G41580 Mustard Weed)
TagsMBPExpressionBacterialMutationE281-A496; VEDL 425-428 AAAPromoterP-lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ING2(pHD)
Plasmid#21589DepositorInsertGFP-ING2(pHD) (Ing2 Mouse)
TagsEGFPExpressionMammalianMutationcontains Ing2 plant homeodomain (PHD) amino acids…Available SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF0341
Plasmid#141584PurposeLentiviral vector for overexpressing the MECOM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCENPTΔC-dCas9-3xGFP
Plasmid#198325PurposeExpresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locusDepositorInsertCENPTΔC (CENPT Human)
UseLentiviralTagsEGFP x 3 and dCas9ExpressionMammalianMutationtruncation - encodes only amino acids 1-375PromoterCMV-TetOAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CHFR-PBZ-eGFP
Plasmid#211581PurposeExpressing CHFR PBZ domain fused to eGFPDepositorInsertCHFR PBZ domain (CHFR Human)
TagseGFPExpressionMammalianMutationPBZ domain of the proteinPromoterCMVAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0340
Plasmid#141583PurposeLentiviral vector for overexpressing the MECOM transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
nCas9-UGI-NLS-P2A-EGFP (pJUL1001)
Plasmid#123611PurposeCAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).DepositorInsertnCas9-UGI-NLS-P2A-EGFP
ExpressionMammalianMutationD10A in SpCas9PromoterCAGAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Anti-DNAH7 [N250/21.4R]
Plasmid#164158PurposeMammalian Expression Plasmid of anti-DNAH7 (Human). Derived from hybridoma N250/21.4.DepositorInsertanti-DNAH7 (Homo sapiens) recombinant mouse monoclonal antibody (DNAH7 Mouse)
ExpressionMammalianPromoterdual CMVAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
L4315 IL-10Ra CTEVp chain in PolyTX-mTagBFP2
Plasmid#244171PurposeConstitutive expression of a MESA CTEVp chain encoding (N to C): IL10RaSS-3xFLAG-IL10RaECD-IL10RaTMD-CTEVp(190K)-PRS(M)-VP64-ZF6aDepositorInsertMESA CTEVp chain with human IL-10Ra SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (IL10RA Human, Synthetic)
UseSynthetic BiologyTagsIL10Ra signal peptide - 3xFLAGExpressionMammalianMutationCTEVp_190KPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4804 VEGFR1 CTEVp chain in PolyTX-mTagBFP2
Plasmid#244174PurposeConstitutive expression of a MESA CTEVp chain encoding (N to C): VEGFR1SS-3xFLAG-VEGFR1ECD-VEGFR1TMD-CTEVp(190K)-PRS(M)-VP64-ZF6DepositorInsertMESA CTEVp chain with human VEGFR1 SS, ECD and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (FLT1 Human, Synthetic)
UseSynthetic BiologyTagsVEGFR1 signal peptide - 3xFLAGExpressionMammalianMutationCTEVp_190KPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4308 IL-10Rb NTEVp chain (human IgG VH SS) in PolyTX-mNeonGreen
Plasmid#244169PurposeConstitutive expression of a MESA NTEVp chain encoding (N to C): hIgGVHSS-3xFLAG-IL10RbECD-IL10RbTMD-NTEVp(75S)DepositorInsertMESA NTEVp chain with human IgG-VH SS, human IL-10Rb ECD and TMD, and NTEVp 75S mutant (IL10RB Human, Synthetic)
UseSynthetic BiologyTagshuman IgG VH signal peptide - 3xFLAGExpressionMammalianMutationNTEVp_75SPromoterhEF1aAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only