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Showing: 3751 - 3800 of 17876 results

  1. KBB609

    Plasmid
    #185110
    Purpose
    Pom152 N-terminus in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 N-term-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185110-plasmid-map-sequence-id-368108

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  2. pRS416-PHO5-GFP-Opi1 + terminator

    Plasmid
    #58836
    Purpose
    Expresses GFP-Tagged Opi1 + Terminator in yeast
    Depositor
    Tim Levine
    Insert
    Opi1p (OPI1 Budding Yeast)
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    PHO5
    Available since
    Sept. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pGAL-SKI1-NAT

    Plasmid
    #160382
    Purpose
    Overxpression of SKI1/XRN1 in yeast
    Depositor
    John McCusker
    Insert
    SKI1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160382-plasmid-map-sequence-id-314520

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  4. LHP462 - Ub-HA

    Plasmid
    #32169
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. pVL1337

    Plasmid
    #239541
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-ScPex15(315-383)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239541-plasmid-map-sequence-id-475879

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  6. Sc FLAG-RFC1-pRS405/GAL-L

    Plasmid
    #239476
    Purpose
    Overexpress Sc FLAG-RFC1 in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239476-plasmid-map-sequence-id-475691

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  7. pOCC240-MBP-Pab1-mCherry

    Plasmid
    #219980
    Purpose
    Pab1p-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    Pab1p-mCherry (PAB1 Budding Yeast)
    Use
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219980-plasmid-map-sequence-id-436810

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  8. pOCC240-MBP-eIF4A-mCherry

    Plasmid
    #219982
    Purpose
    eIF4A-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4A-mCherry (TIF1 Budding Yeast)
    Use
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219982-plasmid-map-sequence-id-436882

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  9. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Matthias Heinemann
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166111-plasmid-map-sequence-id-330321

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  10. pMLLCK-J72038

    Plasmid
    #26183
    Depositor
    Christopher Anderson
    Insert
    Med21 aa1-132 for N-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26183-plasmid-map-sequence-id-12304

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  11. pMLLKA-J72039

    Plasmid
    #26184
    Depositor
    Christopher Anderson
    Insert
    Med7 aa102-205 for C-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    26184-plasmid-map-sequence-id-12305

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  12. pMLLKA-J72040

    Plasmid
    #26185
    Depositor
    Christopher Anderson
    Insert
    Med21 aa1-132 for C-terminal tagging
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2011
    Availability
    Academic Institutions and Nonprofits only

  13. pGR475

    Plasmid
    #213784
    Purpose
    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker
    Depositor
    Chang Liu
    Insert
    WT TP-DNAP1 (recoded)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213784-plasmid-map-sequence-id-424627

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  14. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  15. pPICZac-STE2-1D4

    Plasmid
    #133437
    Purpose
    Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S/C252A
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133437-plasmid-map-sequence-id-267729

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  16. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  17. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. KBB610

    Plasmid
    #185111
    Purpose
    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 Repeats-6His
    Promoter
    Available since
    Aug. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    185111-plasmid-map-sequence-id-368105

    Enlarge

  19. LHP905 - Gal1-Ub

    Plasmid
    #32173
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

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  21. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

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  22. Sc RFC[3+4]-pRS402/GAL

    Plasmid
    #239475
    Purpose
    Overexpress Sc RFC3 & RFC4 in yeast (integrated)
    Depositor
    Michael O'Donnell
    Inserts
    Sc RFC3 (RFC3 Budding Yeast)
    Sc RFC4 (RFC4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239475-plasmid-map-sequence-id-475712

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  23. Sc FLAG-S6-RFC1-pRS405/GAL-L

    Plasmid
    #239473
    Purpose
    Overexpress Sc FLAG-S6-RFC1 in yeast (integrated)
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-S6-RFC1 (RFC1 Budding Yeast)
    Use
    Tags
    3X FLAG followed by S6
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239473-plasmid-map-sequence-id-475709

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  24. Sc RFC[2+5]-pRS403/GAL

    Plasmid
    #239474
    Purpose
    Overexpress Sc RFC2 & RFC5 in yeast (integrated)
    Depositor
    Michael O'Donnell
    Inserts
    Sc RFC2 (RFC2 Budding Yeast)
    Sc RFC5 (RFC5 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239474-plasmid-map-sequence-id-475694

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  25. pAIP4_5H28

    Plasmid
    #234184
    Purpose
    Heterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5H28 (SWH1 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234184-plasmid-map-sequence-id-464149

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  26. pOCC102-MBP-eIF4G-459

    Plasmid
    #219975
    Purpose
    eIF4G-459 protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-459 (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    459LL460 to 459AA460
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219975-plasmid-map-sequence-id-436889

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  27. pOCC120-MBP-Ded1-monoGFP

    Plasmid
    #219983
    Purpose
    Ded1p-monoGFP protein purification
    Depositor
    Simon Alberti
    Insert
    Ded1p-monoGFP (DED1 Budding Yeast)
    Use
    Tags
    MBP and monoGFP-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219983-plasmid-map-sequence-id-436836

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  28. pOCC102-MBP-eIF4G-213

    Plasmid
    #219974
    Purpose
    eIF4G-213 protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-213 (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    213VKLR216 to 213AAAA216
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219974-plasmid-map-sequence-id-436799

    Enlarge

  29. KBB611

    Plasmid
    #185112
    Purpose
    Pom152 central repeats 4 and 5 in pEX-N-His for bacterial expression and purification
    Depositor
    Ken Belanger
    Insert
    POM152
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Pom152 repeats 4&5-6His
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185112-plasmid-map-sequence-id-368078

    Enlarge

  30. LDB223

    Plasmid
    #185428
    Purpose
    Bacterial expression Nup2 N-terminal domain as GST fusion
    Depositor
    Laura Davis, Ken Belanger
    Insert
    NUP2
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup2 N-terminal domain
    Promoter
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185428-plasmid-map-sequence-id-367563

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  39. TK4-EGFP

    Plasmid
    #239047
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    239047-plasmid-map-sequence-id-473421

    Enlarge

  40. pGAPZa-STE2-1D4

    Plasmid
    #133422
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S, C252A
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133422-plasmid-map-sequence-id-267645

    Enlarge

  41. pHflu4

    Plasmid
    #203882
    Purpose
    pHI1 with insertion of monomeric red fluorescent protein (mRFP)
    Depositor
    Bogumil Karas
    Insert
    monomeric red fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203882-plasmid-map-sequence-id-403835

    Enlarge

  42. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

    Enlarge

  45. YEpADE1

    Plasmid
    #22495
    Depositor
    Beth Montelone
    Insert
    ADE1 (ADE1 Budding Yeast)
    Use
    Yeast shuttle
    Tags
    Expression
    Mutation
    Original 4067 bp HindIII-HindIII insertion (see Y…
    Promoter
    Available since
    Jan. 20, 2010
    Availability
    Academic Institutions and Nonprofits only
    22495-plasmid-map-sequence-id-10339

    Enlarge

  46. LHP2691 - pET30-Ub-K63A

    Plasmid
    #32179
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K63A
    Promoter
    T7
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  47. LHP2690 - pET30-Ub-K48A

    Plasmid
    #32178
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    HIS
    Expression
    Bacterial
    Mutation
    K48A
    Promoter
    T7
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 3751 - 3800 of 17876 results