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Plasmid#108474Purposeexpression of vsv-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
UseTagsVSVExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-Mis12-dCEN-Incenp
Plasmid#108477Purposeexpression of vsv-Mis12-dCEN-INCENPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx6
Plasmid#108135PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]6:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]6:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx4
Plasmid#108134PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]4:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]4:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx2
Plasmid#108133PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]2:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-shScn4b-268
Plasmid#99031PurposeExpresses a shRNA targeting the mouse Scn4b geneDepositorInsertScn4b shRNA (Scn4b Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(Q66L)-RagC(Q120L)
Plasmid#99702PurposeBacterial expression of RagA(Q66L)-RagC(Q120L)DepositorUseTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationQ120L and Q66LPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mCherry Gamma 3
Plasmid#65234PurposeTo study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cellDepositorInsertsUseTags6-His tagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC2
Plasmid#72438PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to ArgPromoterT7 and n/aAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sdk2-Fc-His
Plasmid#72135PurposeExpresses the extracellular region of the Sdk2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSdk2 (Sdk2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(S)-Fc-His
Plasmid#72152PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.a (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.z(S)-Fc-His
Plasmid#72153PurposeExpresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.z (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5a.a-AP-His
Plasmid#72050PurposeExpresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5a.a (Unc5a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rgma-Fc-His
Plasmid#72133PurposeExpresses the extracellular region of the RGMa protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertRgma (Rgma Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema4f.a-Fc-His
Plasmid#72158PurposeExpresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema4f.a (Sema4f Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.z(S)-AP-His
Plasmid#72027PurposeExpresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3g.z (Sema3g Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.5-Fc-His
Plasmid#72171PurposeExpresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.5 (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(S)-Fc-His
Plasmid#72144PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/purC
Plasmid#73623PurposeProduces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tagDepositorInsertphosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationContains multiple silent mutations (E179, G185, G…PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6a.z-AP-His
Plasmid#72038PurposeExpresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6a.z (Sema6a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.y-Fc-His
Plasmid#72184PurposeExpresses the extracellular region of the Unc5D, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5d.y (Unc5d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.x-AP-His
Plasmid#72047PurposeExpresses the extracellular region of the Sema6D, isoform x protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.x (Sema6d Human, Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5c.z-AP-His
Plasmid#72056PurposeExpresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5c.z (Unc5c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.6-AP-His
Plasmid#72046PurposeExpresses the extracellular region of the Sema6D, isoform 6 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.6 (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.y-AP-His
Plasmid#72058PurposeExpresses the extracellular region of the Unc5D, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5d.y (Unc5d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.z-AP-His
Plasmid#72048PurposeExpresses the extracellular region of the Sema6D, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.z (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5d.z-AP-His
Plasmid#72059PurposeExpresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertUnc5d.z (Unc5d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.2-AP-His
Plasmid#72043PurposeExpresses the extracellular region of the Sema6D, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema6d.2 (Sema6d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.z-Fc-His
Plasmid#72174PurposeExpresses the extracellular region of the Sema6D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.z (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.x-Fc-His
Plasmid#72173PurposeExpresses the extracellular region of the Sema6D, isoform x protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.x (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema6d.2-Fc-His
Plasmid#72169PurposeExpresses the extracellular region of the Sema6D, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema6d.2 (Sema6d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5a.z-Fc-His
Plasmid#72177PurposeExpresses the extracellular region of the Unc5A, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5a.z (Unc5a Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.b-AP-His
Plasmid#71964PurposeExpresses the extracellular region of the Neogenin 1, isoform b protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.b (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.z-Fc-His
Plasmid#72179PurposeExpresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertUnc5b.z (Unc5b Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(L)-AP-His
Plasmid#72000PurposeExpresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(S)-AP-His
Plasmid#72018PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.f-AP-His
Plasmid#71968PurposeExpresses the extracellular region of the Neogenin 1, isoform f protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.f (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.g-AP-His
Plasmid#71969PurposeExpresses the extracellular region of the Neogenin 1, isoform g protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.g (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.d-AP-His
Plasmid#71966PurposeExpresses the extracellular region of the Neogenin 1, isoform d protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.d (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorUseTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238) in pcDNAI/Amp
Plasmid#60896PurposeThis vector attaches CFP(159-238) to the N-terminus of a protein.DepositorInsertCFP(159-238)
UseTagsExpressionMammalianMutationAsn-164 to HisPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Alpha-0 Dronpa
Plasmid#44193DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsDronpaExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2deltaC
Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a D540K
Plasmid#53057PurposeExpresses alpha subunit of D540K hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationchanged Aspartate 540 to LysinePromoterSP6Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-MRPL15
Plasmid#31345DepositorInsertmitochondrial ribosomal protein L15 mature form (MRPL15 Human)
UseTagsHisExpressionBacterialMutationHas the amino acids in the predicted mature form …PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only