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Viral Prep#154948-AAVrgPurposeReady-to-use AAV Retrograde particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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AAV pCAG-FLEX-EGFP-WPRE (AAV8)
Viral Prep#51502-AAV8PurposeReady-to-use AAV8 particles produced from AAV pCAG-FLEX-EGFP-WPRE (#51502). In addition to the viral particles, you will also receive purified AAV pCAG-FLEX-EGFP-WPRE plasmid DNA. CAG-driven, Cre-dependent EGFP expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEGFP (Cre-dependent)Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SOUL-P2A-tdTomato
Plasmid#153019PurposeStep-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque.DepositorInsertSOUL
UseAAVTagsP2A-tdTomatoExpressionMammalianMutationC128S/D156A/T159CPromoterAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hNPM
Plasmid#181887PurposeSoluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hNPM (NPM1 Mustard Weed, Human)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hNCL
Plasmid#181886PurposeSoluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hNCL (NCL Mustard Weed, Human)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1_LUP1
Plasmid#103857PurposeHeterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertlupeol synthase 1 (LUP1 Mustard Weed)
UseSynthetic BiologyTagsExpressionBacterialMutationinserted genes are codon optimized. 2nd amino aci…PromoterPcoaT (from Synechocystis sp. PCC 6803)Available sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-CIB1-mRuby-Rab35
Plasmid#194334PurposeFor use in light-induced Rab35 protein inactivation through the interaction with CRY2DepositorUseTagsmRubyExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
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RALA D130G
Plasmid#122929PurposeBacterial expression of RALA D130GDepositorInsertRALA (RALA Human)
UseTagsHisExpressionBacterialMutationD130GPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RALA S157A
Plasmid#122930PurposeBacterial expression of RALA S157ADepositorInsertRALA (RALA Human)
UseTagsHisExpressionBacterialMutationS157APromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RALA R176X
Plasmid#122931PurposeBacterial expression of RALA R176XDepositorInsertRALA (RALA Human)
UseTagsHisExpressionBacterialMutationR176XPromoterT7Available sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-mHP1alpha
Plasmid#181883PurposeSoluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-mHP1alpha (Cbx5 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1gamma
Plasmid#181885PurposeSoluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hHP1gamma (CBX3 Mustard Weed, Human)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…Available sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1alpha
Plasmid#181884PurposeSoluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-hHP1alpha (CBX5 Mustard Weed, Human)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-MECP2
Plasmid#181888PurposeSoluble light-dependent effector protein (Methyl-CpG binding protein) for optodroplet formation or recruitment to CIBN localizersDepositorInsertPHR-mCherry-MECP2 (Mecp2 Mustard Weed, Mouse)
UseTagsPHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only