We narrowed to 4,320 results for: Pet2

1. pET28a SnoopTag-mEGFP-SpyTag

   Plasmid

   #72325

   Purpose

   Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-mEGFP-SpyTag
   

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZH81 pET21b Rat1-His

   Plasmid

   #231650

   Purpose

   Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   Rat1 (RAT1 Budding Yeast)
   

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-Nsp14-TEV-Strep

   Plasmid

   #154953

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET28a T7pCONS TIR-1 sfGFP

   Plasmid

   #154463

   Purpose

   pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Tags

   His-thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Tags

   His6 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ICMAB - pET22b T7 EncFtn Mms6

   Plasmid

   #225538

   Purpose

   Construction of intracellular magnetite accumulating bacterial systems.

   Depositor

   Urartu Seker

   Article

   Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

   Inserts

   Iron binding protein
   Ferroxidase
   Material binding peptite
   Iron transporter protein
   

   Tags

   no

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Tags

   6x-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23b-Casp3 C163A-His

   Plasmid

   #11822

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C163A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28a(+) NRP1 b1 (273-427)

   Plasmid

   #162734

   Purpose

   Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1 domain residues 273-427
   

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG94-1--LaG94-14

    Plasmid

    #228973

    Purpose

    For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-1--LaG94-14
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-TEX264-deltaLRR-His6

    Plasmid

    #220215

    Purpose

    Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    c.4-75del / p.2-24del

    Promoter

    T7

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-AviTag-DogTag-MBP

    Plasmid

    #171773

    Purpose

    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogTag-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-cys-His6-OtUBD

    Plasmid

    #190091

    Purpose

    Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b-mEB3-eGFP-6xHis

    Plasmid

    #130983

    Purpose

    Bacterial expression of mouse EB3-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB3-GFP-6xHis (Mapre3 Mouse)
    

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid

    #196972

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SENP2 (catalytic domain)

    Plasmid

    #16357

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP2 catalytic domain (SENP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 365 to 590 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21 Catalytic Domain of MALT1

    Plasmid

    #48970

    Purpose

    Expresses catalytic domain of MALT1 in e.coli AA 329-566

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 (MALT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains AA 329-566

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only