We narrowed to 1,731 results for: plasmids pcas

Showing: 1501 - 1520 of 1731 results

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pcDNA3.1 Cas9-P2A-dTomato-T20

Plasmid

#231912

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pWN10042

Plasmid

#89052

Purpose

GF-ori-FP plasmid for GFxFP assay

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

EGFP part 1
EGFP part 2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and none

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT46_GalL_cCDA1-BE3

Plasmid

#145039

Purpose

Expresses cCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

cCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pJT171_GalL_A3AΔ178-BE3

Plasmid

#145121

Purpose

Expressing base editor A3AΔ178-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ178-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-178AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT170_GalL_A3AΔ182-BE3

Plasmid

#145120

Purpose

Expressing base editor A3AΔ182-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ182-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-182AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT169_GalL_A3AΔ186-BE3

Plasmid

#145119

Purpose

Expressing base editor A3AΔ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3AΔ186-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(1-186AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT92_GalL_nCDA1Δ188-BE3

Plasmid

#145052

Purpose

Expresses nCDA1Δ188-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ188-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-188AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT90_GalL_nCDA1Δ190-BE3

Plasmid

#145050

Purpose

Expresses nCDA1Δ190-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ190-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-190AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT85_GalL_nCDA1Δ193-BE3

Plasmid

#145048

Purpose

Expresses nCDA1Δ193-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ193-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-193AA); spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT84_GalL_nCDA1Δ194-BE3

Plasmid

#145047

Purpose

Expresses nCDA1Δ194-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ194-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-194AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT78_GalL_nCDA1Δ195-BE3

Plasmid

#145044

Purpose

Expresses nCDA1Δ195-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ195-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-195AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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epi-BE4max-NG

Plasmid

#135977

Purpose

Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only