161,398 results

1. pEIGW roGFP2-Orp1

   Plasmid

   #64993

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-CAG-IRF4-FLAG-IRES-GFP

   Plasmid

   #107389

   Purpose

   Lentiviral expression of IRF4-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-puro /BRAF-V600E

   Plasmid

   #131724

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.GCaMP6s.WPRE.SV40

   Plasmid

   #100843

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3XMEF2-luc

   Plasmid

   #32967

   Purpose

   Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

   Depositor

   Ron Prywes

   Insert

   MEF2 binding sites (MEF2A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   MEF2

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-FLPo

   Plasmid

   #118027

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-DIO-FLPo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV09_pCMV-ADAR1-p110

   Plasmid

   #200404

   Purpose

   Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   ADAR-p110
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-DJ-N589X

   Plasmid

   #130878

   Purpose

   Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

   Depositor

   Prashant Mali

   Article

   Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

   Insert

   AAV-DJ cap N589X
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N589X

   Promoter

   Unknown

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-2E2-HA-mCh

    Plasmid

    #129596

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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