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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,404 results for: Ada;

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Showing: 7661 - 7670 of 10404 results

  1. Cherry-HA-KCC2

    Plasmid
    #104077
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Tags
    mCherry-HA tag
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104077-plasmid-map-sequence-id-199357

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  3. pDEST-Flag-FBW7K404R

    Plasmid
    #200717
    Purpose
    The plasmid expresses Flag-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for mammalian overexpression and detection by western blotting.
    Depositor
    Omar Khan
    Article
    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:
    Insert
    FBW7 (FBXW7 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    FBW7 K404 is mutated to Alanine.
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200717-plasmid-map-sequence-id-394909

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  6. pCW-ND-Cdt1-mCherry-Puro

    Plasmid
    #193766
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193766-plasmid-map-sequence-id-380221

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  8. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid
    #105810
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Available Since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105810-plasmid-map-sequence-id-204854

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  10. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Article
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available Since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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Showing: 7661 - 7670 of 10404 results