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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,323 results for: NSI

Showing: 3041 - 3080 of 12323 results

  3. pBP-C_ccdB

    Plasmid
    #204114
    Purpose
    Type 0 CDS part
    Depositor
    Mikhail Shapiro
    Insert
    Toxic DNA gyrase inhibitor ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204114-plasmid-map-sequence-id-418238

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  7. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  8. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  9. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  11. pJBL7068

    Plasmid
    #167237
    Purpose
    His-tagged sfGFP for protein purification
    Depositor
    Julius Lucks
    Insert
    pT7-6XHis-sfGFP-MGA
    Use
    Synthetic Biology
    Tags
    6X His
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    167237-plasmid-map-sequence-id-331130

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  12. pBP-Tag_phoA

    Plasmid
    #204107
    Purpose
    Type 0 tag part
    Depositor
    Mikhail Shapiro
    Insert
    Signal peptide from E. coli PhoA protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    204107-plasmid-map-sequence-id-437314

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  22. pBP-T_his-min-S

    Plasmid
    #204123
    Purpose
    Type 0 terminator part
    Depositor
    Mikhail Shapiro
    Insert
    his-min-S strong terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204123-plasmid-map-sequence-id-418256

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  23. pBP-Tag_dsbA

    Plasmid
    #204106
    Purpose
    Type 0 tag part
    Depositor
    Mikhail Shapiro
    Insert
    Signal peptide from E. coli DsbA protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204106-plasmid-map-sequence-id-418230

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  24. pTU2-b_CrK_amilCP

    Plasmid
    #204067
    Purpose
    Type TU2-b backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204067-plasmid-map-sequence-id-418202

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  25. pTU2-b_UrK_amilCP

    Plasmid
    #204065
    Purpose
    Type TU2-b backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204065-plasmid-map-sequence-id-418194

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  26. pTU1-A_CrK_amilCP

    Plasmid
    #204046
    Purpose
    Type TU1-A backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204046-plasmid-map-sequence-id-418163

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  27. pTU1-A_UrK_amilCP

    Plasmid
    #204044
    Purpose
    Type TU1-A backbone with kanamycin resistance and pUC-bom-rop ORI. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204044-plasmid-map-sequence-id-418162

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  28. pGL2_213

    Plasmid
    #210697
    Purpose
    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion
    Depositor
    Cultivarium Tools, Nili Ostrov, Charlie Gilbert
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210697-plasmid-map-sequence-id-417392

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  29. pGL2_212

    Plasmid
    #210696
    Purpose
    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion
    Depositor
    Cultivarium Tools, Nili Ostrov, Charlie Gilbert
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210696-plasmid-map-sequence-id-417391

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  30. pGL2_211

    Plasmid
    #210695
    Purpose
    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion
    Depositor
    Cultivarium Tools, Nili Ostrov, Charlie Gilbert
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    210695-plasmid-map-sequence-id-417388

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  31. U22m

    Plasmid
    #121025
    Purpose
    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121025-plasmid-map-sequence-id-245900

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  32. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

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  33. pBP-P_tetR-PTet*

    Plasmid
    #204077
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    tetR repressor and PTet* tetracycline-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204077-plasmid-map-sequence-id-413892

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  35. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

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  36. pTU2-a_SKA_amilCP

    Plasmid
    #204055
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101 (WT) ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204055-plasmid-map-sequence-id-413881

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  37. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

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  38. C11m

    Plasmid
    #120959
    Purpose
    MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Citrine
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120959-plasmid-map-sequence-id-238041

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  39. C5m

    Plasmid
    #120957
    Purpose
    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).
    Depositor
    Richard Murray
    Insert
    mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120957-plasmid-map-sequence-id-236154

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  40. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

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Showing: 3041 - 3080 of 12323 results