We narrowed to 4,431 results for: PGK

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Publication

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67290

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Publication

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-GFP-PGK-Puro

Plasmid

#110867

Purpose

Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Publication

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG::Flcn-pgk-hph

Plasmid

#48757

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection

Depositor

Austin Smith

Publication

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Flcn (Flcn Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p67.uni.pA.OTIIp.mHEL.flag.mCMV.PGK.CD63GFP.WPRE

Plasmid

#228765

Purpose

mHEL and OT2-peptide

Depositor

Ferdinando Pucci

Insert

OVA epitope ISQAVHAAHAEINEAGR

Expression

Mammalian

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R

Plasmid

#208091

Purpose

Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Publication

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

none

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-hPAX8_tdTomato-PGK-PuroTK

Plasmid

#206004

Purpose

plasmid to insert tdTomato reporter at the 3' end of the human PAX8 gene

Depositor

Darrell Kotton

Publication

Undeutsch et al Stem Cell Reports. 2024 Dec 10;19(12):16

Inserts

PAX8 5' homology arm, P2A-tdTomato (PAX8 Human)
lox-P - PGK - puroTK - loxP
PAX8 3' homology arm (PAX8 Human)

Expression

Mammalian

Promoter

PGK

Available since

Oct. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK)-12xHRE

Plasmid

#172334

Purpose

hypoxic responsive reporter

Depositor

David Bersten

Publication

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

PGK

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-WT-neo

Plasmid

#156173

Purpose

Lentiviral vector for expression of RAP1A-WT, with neomycin section

Depositor

Roland Friedel

Publication

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

Plasmid

#167819

Purpose

control sensor for EKAREN5

Depositor

Hugo Snippert

Publication

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EKAREN5(TA)

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W, T420A

Promoter

EF1a

Available since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-SOX2

Plasmid

#115687

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Publication

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MD-SOX2

Use

Lentiviral

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-BE3RA-PGK-Puro

Plasmid

#110843

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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SIN-PGK-Cas9-WPRE

Plasmid

#87886

Purpose

To produce Lentiviral vector for gene editing

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

hCas9

Use

Lentiviral

Tags

nls

Mutation

Human codon-optimized

Promoter

mouse PGK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Publication

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Flag-MFF-S155,172E

Plasmid

#74443

Purpose

Lentiviral expression vector containing flag tagged S155,172E MFF isoform 5 mutant

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Glu, Ser 172 Glu (Numbering based on MFF …

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
pHR-PGK-WP1-CRY2PHR-VP64

Plasmid

#171758

Purpose

Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64

Depositor

Yingxiao Wang

Publication

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

WP1-CRY2PHR-VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-E63-neo

Plasmid

#156174

Purpose

Lentiviral vector for expression of RAP1A-E63, with neomycin selection

Depositor

Roland Friedel

Publication

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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