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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,787 results for: emb

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Showing: 1521 - 1540 of 16787 results

  1. pHLSec2-FN-YPet

    Plasmid
    #65421
    Purpose
    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
    Depositor
    Harold Erickson
    Publication
    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    Tags
    YPet
    Expression
    Mammalian
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65421-plasmid-map-sequence-id-115435

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  3. pRluc-LC3G120A

    Plasmid
    #105003
    Purpose
    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.
    Depositor
    Marja Jããttelã
    Publication
    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LC3B (Map1lc3b Rat)
    Use
    Luciferase
    Tags
    Rluc with C124A substitution
    Expression
    Mammalian
    Mutation
    Glycine 120 mutated to alanine, prevents lipidati…
    Available since
    Jan. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105003-plasmid-map-sequence-id-202164

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  7. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Publication
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87381-plasmid-map-sequence-id-212242

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  10. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Publication
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

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  11. pcDNA-LBR-EGFP-polyA

    Plasmid
    #59748
    Purpose
    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LBR-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59748-plasmid-map-sequence-id-94052

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  15. pcDNA3.1 CARMA1

    Plasmid
    #44431
    Depositor
    Xin Lin
    Insert
    CARMA1 (CARD11 Human)
    Expression
    Mammalian
    Mutation
    See depositor comments below.
    Available since
    Oct. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44431-plasmid-map-sequence-id-263620

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  18. EHD2-mEGFP

    Plasmid
    #45932
    Purpose
    caveolar protein, confines caveolae to the plasma membrane
    Depositor
    Ari Helenius
    Publication
    Stoeber et al EMBO J. 2012 May 16;31(10):2350-64. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eps-15 homology domain-containing protein2 (EHD2 Human)
    Tags
    monomeric EGFP_FrameN1
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45932-plasmid-map-sequence-id-66945

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  19. pEGFPC1-hVAP-A KD/MD

    Plasmid
    #104449
    Purpose
    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    vesicle-associated membrane protein-associated protein A (VAPA Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed K94 to D and M96 to D
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104449-plasmid-map-sequence-id-201409

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Showing: 1521 - 1540 of 16787 results