We narrowed to 6,252 results for: nls

1. pHR-RUNX2-IDR-mCherry-CRY2-NLS

   Plasmid

   #145267

   Purpose

   Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   RUNX2wt-IDR (RUNX2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

   Plasmid

   #134331

   Purpose

   CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized Nme2Cas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

   Plasmid

   #123611

   Purpose

   CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   nCas9-UGI-NLS-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2ADW-lox-mCherry-hyBRET-ERK-NLS

   Plasmid

   #170290

   Purpose

   For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK.

   Depositor

   Michiyuki Matsuda

   Article

   Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

   Insert

   lox-mCherry-lox-hyBRET-ERK-NLS
   

   Expression

   Mammalian

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG-Flag-Sox9-IRES-nls-GFP

   Plasmid

   #44281

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   Flag-Sox9 (SOX9 Chicken)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV-IE enhancer and Chicken beta-actin promoter

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

   Plasmid

   #31478

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV HA NLS Y2 K227M T2A BFP
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   F13Y, S111Y, K227M = Y2 nickase "reo" …

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

   Plasmid

   #85844

   Purpose

   Genetically encoded voltage sensor ArcLight- codon optimized

   Depositor

   Vincent Pieribone

   Article

   Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

   Insert

   ArcLightCo-Q239
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   hSyn1

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

   Plasmid

   #127360

   Purpose

   gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   R118G

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169352

    Purpose

    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Insert

    OsTIR1(WT)
    

    Expression

    Yeast

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEG302 22aa SunTag NtDRMcd (no NLS) SUP

    Plasmid

    #115490

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid

    #109429

    Purpose

    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

    Plasmid

    #63060

    Purpose

    Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    nls-mRuby2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. XLone-Puro Cas13d P2A eGFP-NLS

    Plasmid

    #155182

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A eGFP NLS

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB001 CMV 3xFLAG-NLS-FKBP-ZF2

    Plasmid

    #161545

    Purpose

    Constitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NLS-FKBP-ZF2
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid

    #140030

    Purpose

    Tunable and temporal expression control of NFIL3 and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 (NFIL3 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. XE70 NLS Beta-catenin GFP in CS2+

    Plasmid

    #16838

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

    Insert

    NLS beta-catenin GFP (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

18. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

    Plasmid

    #145263

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+9A)-IDR (HOXD13 Human)
    

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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