Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,027 results for: D10

Clear keywords
Showing: 1541 - 1560 of 2027 results

  3. L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen

    Plasmid
    #244173
    Purpose
    Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(WT)
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (WT) (KDR Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    VEGFR2 signal peptide - 3xFLAG
    Expression
    Mammalian
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244173-plasmid-map-sequence-id-487912

    Enlarge

  5. pD649-HAsp-TNFRSF9-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156556
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TNFRSF9 (TNFRSF9 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156556-plasmid-map-sequence-id-368530

    Enlarge

  12. pD649-HAsp-SEMA4D-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156952
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA4D (SEMA4D Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156952-plasmid-map-sequence-id-478926

    Enlarge

  13. pD649-HAsp-SEMA7A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156922
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SEMA7A (SEMA7A Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156922-plasmid-map-sequence-id-502718

    Enlarge

  15. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

    Enlarge

  16. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129036
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129036-plasmid-map-sequence-id-256165

    Enlarge

  17. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129029
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129029-plasmid-map-sequence-id-256133

    Enlarge

  19. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Article
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

    Enlarge

  20. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

    Enlarge

Showing: 1541 - 1560 of 2027 results