We narrowed to 2,925 results for: D10

1. E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

   Plasmid

   #89373

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

   Plasmid

   #89370

   Purpose

   Vector for reporter assay of one zebrafish super-enhancer region

   Depositor

   Alena Shkumatava

   Article

   Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

   Insert

   irf2bpl (irf2bpl Zebrafish)
   

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-NDi-CRISPRi (Gen1)

   Plasmid

   #73497

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB-P2A-mCherry
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-xF2X-P2A-Puro

   Plasmid

   #110873

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS-2X(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRIG-myc-Sema4D-CD4

   Plasmid

   #51606

   Purpose

   expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

   Depositor

   Suzanne Paradis

   Article

   Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

   Insert

   Sema4D/CD4-FLAG (Sema4d Human, Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG and myc

   Expression

   Mammalian

   Mutation

   Sema4D amino acids 660-861 were replaced with CD4…

   Promoter

   RSV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-OptoSTIM1(Cry2(D387A))

   Plasmid

   #70160

   Purpose

   Light-insensitive mutant of OptoSTIM1

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1(Cry2(D387A)) (STIM1 Mustard Weed, Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2K7bsdUBI-mCherry-STIM1

   Plasmid

   #114178

   Purpose

   Lentiviral vector for expression of mCherry-STIM1

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry (inserted after signal peptide)

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

   Plasmid

   #42651

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 399 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T399I

   Promoter

   CMV/TO

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF-His-mTET1CD∆cat

    Plasmid

    #81054

    Purpose

    bacterial expression of catalytic dead catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057 with histid…

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1a-mCherry-STIM1

    Plasmid

    #114176

    Purpose

    Gateway entry clone containing mCherry-STIM1

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Gateway entry clone

    Tags

    mCherry (inserted after signal peptide)

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-TNT-CFP-STIM1-C227W

    Plasmid

    #68403

    Purpose

    Expresses a STIM1 gain-of-function mutant in mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    CFP-STIM1 (STIM1 Human)
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Mutation

    Changed Cysteine 227 to Tryptophan; CFP was inser…

    Promoter

    CMV

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available Since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2

    Plasmid

    #24419

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    RS1 (HTR4 Human)
    

    Use

    Mouse Targeting

    Tags

    FLAG and mCherry

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. hu-TLR4 299 snp-flag pDEST40

    Plasmid

    #42647

    Purpose

    destination vector for expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

18. attB-GFP-Tet1d834-1053-Poly(A)

    Plasmid

    #65561

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    del(834-1053)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-HDAC2-H141A

    Plasmid

    #115346

    Purpose

    Expresses mouse HDAC2 cDNA with H141A point mutation

    Depositor

    Javier Gonzalez-Maeso

    Article

    Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

    Insert

    Histone deacetylase 2 (Hdac2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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