We narrowed to 2,364 results for: PUC19

1. pILGFPU0 (R)

   Plasmid

   #83562

   Purpose

   Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS021

   Plasmid

   #207917

   Purpose

   Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKE013

   Plasmid

   #207916

   Purpose

   Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT15485_Hypa-ABE8e

   Plasmid

   #174123

   Purpose

   Expresses ABE8e fused with Hypa-SpCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   Hypa-ABE8e
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMG

   Plasmid

   #138264

   Purpose

   Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Inserts

   eGFP flanked by iCas9 sites
   mCherry
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tef1

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pITGFP89

   Plasmid

   #83560

   Purpose

   Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TEF1 promoter -yEGFP-CLN2PEST
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVC-CRISPR1

   Plasmid

   #120366

   Purpose

   Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Insert

   CRISPR1
   

   Use

   CRISPR

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVC-CRISPR

   Plasmid

   #120369

   Purpose

   Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Inserts

   CRISPR1
   CRISPR2
   

   Use

   CRISPR

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP9E3

   Plasmid

   #185842

   Purpose

   Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP86

    Plasmid

    #83543

    Purpose

    Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL4A promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    RPL4A

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTX1-EV71-SL2

    Plasmid

    #126043

    Purpose

    In-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    Enterovirus 71 5'UTR SL2
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pADH118-2

    Plasmid

    #90984

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT9993-Hypa-ABE

    Plasmid

    #163001

    Purpose

    plasmid expressing Hypa-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-a

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP84

    Plasmid

    #83542

    Purpose

    Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    SSA1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN144

    Plasmid

    #91673

    Purpose

    Express sgRNA targeting human TBC1D5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TBC1D5 (TBC1D5 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN145

    Plasmid

    #91674

    Purpose

    Express sgRNA targeting human TBC1D5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    TBC1D5 (TBC1D5 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pADH118-17

    Plasmid

    #91727

    Purpose

    NAT-marked C. albicans URA3-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2of2, pSNR52, C. albicans URA3-specific gRNA, LEU2 2of2
    

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pADH143-2

    Plasmid

    #90990

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pADH100-2

    Plasmid

    #90981

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKH9-catP

    Plasmid

    #244450

    Purpose

    A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a thiamphenicol resistance marker (catP gene).

    Depositor

    Xue Liu

    Inserts

    pKH09 Fn ori
    CatP
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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