We narrowed to 2,492 results for: PUC19
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Plasmid#185894PurposeIn vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRTetR-GAL80
Plasmid#185853PurposePlugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiaeDepositorInsertgal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
ExpressionYeastMutationNoneAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-460 tNGFR-P2A-STAT4
Plasmid#186075PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDY153: Flag(SUZ12) WT HDR plasmid
Plasmid#170793PurposeWT homology directed repair plasmid for performing genome-editing on the SUZ12 LocusDepositorInsertSUZ12 (SUZ12 Human)
ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZBTB12-donor
Plasmid#113802PurposeCRISPR donor plasmid to tag human transcription factor ZBTB12 with GFPDepositorInsertZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
UseCRISPRMutationrs9267664, rs9267663Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUSF2-donor
Plasmid#112361PurposeCRISPR donor plasmid to tag human transcription factor USF2 with GFPDepositorInsertUSF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
UseCRISPRMutationhg38:19:35278400:T>A, hg38:19:35278455:G>CAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXD1-donor
Plasmid#112350PurposeCRISPR donor plasmid to tag human transcription factor MXD1 with GFPDepositorInsertMXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
UseCRISPRMutationhg38:2:69937537:A>C,rs3771530Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZBTB11-donor
Plasmid#112348PurposeCRISPR donor plasmid to tag human transcription factor ZBTB11 with GFPDepositorInsertZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
UseCRISPRMutationhg38:3:101651181:A>TAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDLX1-donor
Plasmid#112343PurposeCRISPR donor plasmid to tag human transcription factor DLX1 with GFPDepositorInsertDLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
UseCRISPRMutationhg38:2:172087403:G>C,hg38:2:172087433:G>A,h…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRMutationhg38:4:55435551:A>G, hg38:4:55435749:G>AAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
ExpressionYeastPromoterGAL7Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ3
Plasmid#83558PurposeUsed to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.DepositorInsertGAL2 promoter-yEGFP
ExpressionYeastPromoterGAL2Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
WT FLAG-HaloTag-RTEL1_DonorVector
Plasmid#247170PurposeHR Donor for WT C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.DepositorInsertWT FLAG-HaloTag-RTEL1 (RTEL1 Human)
PromoterEndogenous RTEL1 promoterAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIN1 (gpdA-cTerm-mGL-ptrA)
Plasmid#188735PurposeExpresses mGreenlantern for C-terminal taggingDepositorInsertgdpA-mGreenlantern PtrA
UseFilamentous fungal expressionTagsmGreenlanternPromotergpdAAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-MAPRE1
Plasmid#227324PurposeDonor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)DepositorInsertMAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only