We narrowed to 6,752 results for: hwa

1. pCSJ182

   Plasmid

   #159723

   Purpose

   Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   GID4 (VID24 Budding Yeast)
   

   Use

   Yeast two-hybrid assay

   Tags

   3xflag and Gal4AD

   Expression

   Yeast

   Mutation

   remove stop codon

   Promoter

   ADH1

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSJ392

   Plasmid

   #159722

   Purpose

   Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

   Depositor

   Alexander Varshavsky

   Article

   Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

   Insert

   GID10 (GID10 Budding Yeast)
   

   Use

   Yeast two-hybrid assay

   Tags

   3xflag and Gal4AD

   Expression

   Yeast

   Mutation

   remove stop codon

   Promoter

   ADH1

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM45

   Plasmid

   #154152

   Purpose

   Firefly luciferase with no promoter for negative control for luciferase assays

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWDY1226

   Plasmid

   #138593

   Purpose

   Expresses NatL2-R156A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Arginine 156 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWDY1222

   Plasmid

   #138592

   Purpose

   Expresses NatL2-K418A in E.coli

   Depositor

   James Naismith

   Article

   Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

   Insert

   phenylacetate-coenzyme A ligase
   

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   changed Lysine 418 to Alanine

   Promoter

   T7

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-lyzC-RFP-miR-30 backbone

   Plasmid

   #97147

   Purpose

   As a control of miRNA expression

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. KG#80

   Plasmid

   #110875

   Purpose

   Expresses the C. elegans acy-1 cDNA in body wall muscle

   Depositor

   Kenneth Miller

   Article

   Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

   Inserts

   myo-3 promoter
   acy-1 cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Expression

   Bacterial

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pMT dati MCS circfirefly_1100

   Plasmid

   #110117

   Purpose

   Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pMT dati MCS circfirefly_900

   Plasmid

   #110116

   Purpose

   Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Firefly luciferase
   

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hy_pMT dati MCS circfirefly_700

    Plasmid

    #110115

    Purpose

    Expresses a 700-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hy_pMT dati MCS circfirefly_500

    Plasmid

    #110114

    Purpose

    Expresses a 500-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P3nmt1-mCFP

    Plasmid

    #105136

    Purpose

    Yeast gene targeting

    Depositor

    Thomas Pollard, Jian-Qiu Wu

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P3nmt1-mCFP
    

    Tags

    3nmt1-mCFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    3nmt1

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-kanMX6-P3nmt1-mYFP

    Plasmid

    #105138

    Purpose

    Yeast gene targeting

    Depositor

    Thomas Pollard, Jian-Qiu Wu

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    kanMX6-P3nmt1-mYFP
    

    Tags

    3nmt1-mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    3nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS_gpyrG2

    Plasmid

    #90277

    Purpose

    (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg2)
    

    Use

    A. niger

    Expression

    Bacterial

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. P0050::rpaAD53A

    Plasmid

    #101831

    Purpose

    drives expression of rpaA D53A variant from an rpaA-independent promoter

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
    

    Use

    Cloning vector

    Mutation

    D53 mutated to A

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90285

    Purpose

    BB2 with split pyrG marker for homologous integration with linker AB (negative control)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90286

    Purpose

    BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90287

    Purpose

    BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

    Plasmid

    #90288

    Purpose

    BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    pyrGtrunc
    

    Expression

    Bacterial

    Mutation

    truncated version of pyrG gene (sequence is from …

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGE63

    Plasmid

    #79445

    Purpose

    recipient plasmid [single nuclease]; p35S:Cas9, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB (ccdB(letD) )
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    BsaI site eliminated

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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