We narrowed to 1,761 results for: mcherry expressing plasmid

1. mGDF8 donor CFP-hDF

   Plasmid

   #72590

   Purpose

   Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. hGDF8 donor CFP-hDF

   Plasmid

   #72591

   Purpose

   Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

   Depositor

   Renzhi Han

   Article

   Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AP2u2-CMV-S_protein

   Plasmid

   #226456

   Purpose

   Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

   Insert

   Spike Protein
   

   Use

   Tags

   6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

   Plasmid

   #37755

   Purpose

   Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-TdTomato
   

   Use

   AAV and Cre/Lox; Cre-on

   Tags

   TdTomato

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-FAS-TdTomato-WPRE-pA

   Plasmid

   #37092

   Purpose

   Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-FAS-EGFP-WPRE-pA

   Plasmid

   #37091

   Purpose

   Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   d1eGFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2_RFP-60A

   Plasmid

   #200394

   Purpose

   Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. RINS1(mut)

    Plasmid

    #107291

    Purpose

    Plasmid for the expression of the inactive mutant of RINS1 sensor

    Depositor

    Dmytro Yushchenko

    Article

    Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

    Insert

    RINS1(mut)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    two Arg/Ser mutations at the beginning and in the…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-FLEX-Chrimson-TdTomato

    Plasmid

    #209202

    Purpose

    Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics

    Depositor

    Larry Zweifel

    Article

    Soden et al Nat Neurosci. 2020 Aug;23(8):968-980. do

    Insert

    Chrimson
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCggRO-reporter

    Plasmid

    #124582

    Purpose

    Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    CggR promoter element
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

    Plasmid

    #197687

    Purpose

    plasmid for expressing the DNMT3bCD

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI

    Plasmid

    #202596

    Purpose

    plasmid for expressing the DNMT3bCI (catalytic inactive)

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Mutation

    P656V, C657D

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP114

    Plasmid

    #120799

    Purpose

    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Insert

    Pxyl::mCherryOpt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

    Promoter

    Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE-BI-TRIM21

    Plasmid

    #207838

    Purpose

    Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    TRIM21 (TRIM21 Human)
    mCherry
    hIgG1-FC
    VHH-GFP4
    

    Use

    Tags

    weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE-BI-osTIR1

    Plasmid

    #207840

    Purpose

    Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    osTIR
    mCherry
    mAID_-VHH-GFP4
    

    Use

    Tags

    3X HA-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.U6gT28.4.hSyn.H2B.RFP

    Plasmid

    #170387

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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