We narrowed to 2,320 results for: pcas9

1. p2T-CMV-BE4maxCP1028-BlastR

   Plasmid

   #152993

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   BE4-nSpCas9 CP1028-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2T-CMV-CDAmax-BlastR

   Plasmid

   #152995

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   CDA-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EC64422

   Plasmid

   #191768

   Purpose

   CRISPR mutagenesis in Medicago truncatula

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NPM1_mNeonGreen_Donor

   Plasmid

   #178093

   Purpose

   HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   NPM1-mNeonGreen HDR donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LMNA_mScarlet-I_Donor

   Plasmid

   #178092

   Purpose

   HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA-mScarlet-I HDR donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH-A3A-PBE

   Plasmid

   #119774

   Purpose

   Conversion of C to T in rice plants transformation

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Use

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   maize Ubiquitin-1

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2T-CMV-eA3Amax-BlastR

   Plasmid

   #152997

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   eA3A-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   HAM34 and RPL41

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A3A-Gam

    Plasmid

    #119769

    Purpose

    Conversion of C to T monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid

    #74705

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. xABEmax

    Plasmid

    #119813

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ABE8r-R153del

    Plasmid

    #208296

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-R153del-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. epi-BE4max-NG

    Plasmid

    #135977

    Purpose

    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CP1028-CBEmax

    Plasmid

    #119802

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1028) -UGI-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CP1041-ABEmax

    Plasmid

    #119808

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT44c

    Plasmid

    #138511

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9-UGI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge