We narrowed to 1,719 results for: plasmid for e coli

1. pET30a-SARS-CoV2-ORF8

   Plasmid

   #165089

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET26b_AT118-A-V5-His

   Plasmid

   #222849

   Purpose

   E. coli periplasmic expression plasmid encoding Nb. AT118-A, a nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-A
   

   Use

   Tags

   V5-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-DEST42-ARL4D-V5-His6

   Plasmid

   #67426

   Purpose

   Expression of hArl4D with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4D (ARL4D Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   bp 272 A to C; N91T mutation; bp 327 T to G; sil…

   Promoter

   T7/lacO

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDGB1alpha2_KanR_BastaR_Rb7_GFP

   Plasmid

   #186427

   Purpose

   optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

   Depositor

   Lukas Fischer

   Article

   Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

   Inserts

   KanR
   BastaR
   Rb7
   eGFP
   

   Use

   Synthetic Biology; Binary vector for escherichia …

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   CaMV 35S and Pnos

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExp-His-zBasic-RBD

   Plasmid

   #194999

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli

   Depositor

   Marko Hyvönen

   Article

   Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   His8-Zbasic

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mCherry-Nanobody (LaM-2)

   Plasmid

   #162276

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   mCherry-Nanobody (LaM-2)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBR322-TIMER

   Plasmid

   #103056

   Purpose

   medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. strep-IRF8

   Plasmid

   #102872

   Purpose

   Express strep-tagged mouse IRF8 protein in E. coli

   Depositor

   Gary Stormo

   Article

   Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

   Insert

   IRF8 (Irf8 Mouse)
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hisSUMO-CTCF(F1-F9)

    Plasmid

    #102859

    Purpose

    Express DNA binding domain of mouse CTCF in E. coli

    Depositor

    Gary Stormo

    Article

    Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

    Insert

    CTCF (Ctcf Mouse)
    

    Use

    Tags

    hisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ALFY-FYVE 1G

    Plasmid

    #197045

    Purpose

    Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

    Insert

    ALFY (WDFY3 Human)
    

    Use

    Tags

    6xHis-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD71Cas10.1-Pct5.1-crRNA(dadR)

    Plasmid

    #191634

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPPC027.306

    Plasmid

    #171149

    Purpose

    Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLei-sfGFP-D134*

    Plasmid

    #191110

    Purpose

    An E. coli sfGFP reporter with one TAG at position 134

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing D134 to TAG

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLei-sfGFP-V2*D134*

    Plasmid

    #191111

    Purpose

    An E. coli sfGFP reporter with two TAG at positions 2 & 134

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing V2 & D134 to TAG

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-VHH-ddFLN4-HIS-ybbR

    Plasmid

    #169137

    Purpose

    E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

    Depositor

    Michael Nash

    Article

    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

    Insert

    VHH
    

    Use

    Tags

    6x His tag, ddFLN4 tag, and ybbr tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)

    Plasmid

    #191641

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔDBD

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)

    Plasmid

    #191642

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    ΔTM

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge