We narrowed to 15,619 results for: plasmids 101

1. RIPK4 gRNA (BRDN0001146610)

   Plasmid

   #75971

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RIPK4

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RIPK4 (RIPK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CDK3 gRNA (BRDN0001144858)

   Plasmid

   #76766

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK3 (CDK3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PIK3R5 gRNA (BRDN0001145982)

   Plasmid

   #75758

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3R5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3R5 (PIK3R5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PIK3R5 gRNA (BRDN0001146332)

   Plasmid

   #75759

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3R5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3R5 (PIK3R5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PIK3R5 gRNA (BRDN0001146461)

   Plasmid

   #75760

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3R5

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3R5 (PIK3R5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TLK2 gRNA (BRDN0001146448)

   Plasmid

   #75615

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TLK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TLK2 (TLK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TLK2 gRNA (BRDN0001147069)

   Plasmid

   #75616

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TLK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TLK2 (TLK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TLK2 gRNA (BRDN0001147939)

   Plasmid

   #75617

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TLK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TLK2 (TLK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMARO

   Plasmid

   #178086

   Purpose

   Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

   Depositor

   Sören Abel

   Article

   Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

   Insert

   TcR (tetA/tetR)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tetracycline resistance cassette is flanked by Ec…

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3JZN

    Plasmid

    #60123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EED:APC056-C06:C32050 (EED EED:APC056-C06:C32050, Human)
    

    Use

    Tags

    6xHis and GST

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. CDK2_HUMAN_D0

    Plasmid

    #79726

    Purpose

    This plasmid encodes the kinase domain of CDK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. 4MVT

    Plasmid

    #60101

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pB2H2*

    Plasmid

    #163834

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    TCPTP R222M

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-HA-DmMe31B-dsRNAres_I

    Plasmid

    #146528

    Purpose

    Insect Expression of DmMe31B-dsRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmMe31B-dsRNAres (me31B Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation A1101G compared to the sequen…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB2H12*

    Plasmid

    #163838

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    PTPN12 Y64A

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB2H6*

    Plasmid

    #163836

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    SHP1 R459M

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB2Hopt*

    Plasmid

    #163831

    Purpose

    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B(C215S)

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    PTP1B C215S

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB2HoptX

    Plasmid

    #163832

    Purpose

    Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B

    Depositor

    Jerome Fox

    Insert

    SpecR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    MidT Substrate Y4F

    Promoter

    Pro1;ProD;pLacZ-Opt

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

    Plasmid

    #115161

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

    Inserts

    PTRE - tet activator responsive promoter
    flex sequence
    hM3dq-mCherry (inverted)
    flex sequence
    

    Use

    AAV

    Tags

    hM3Dq-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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