We narrowed to 5,484 results for: 129

1. pGEX-2T-RhoA-wt

   Plasmid

   #12959

   Depositor

   Gary Bokoch

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pRDAX-293 C-Tag GFP[1] L10 {Esp3I(2x)} mCherry

   Plasmid

   #129882

   Purpose

   PPI (BiFC/TriFC) assay vector

   Depositor

   Gary Lewin

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-Basic-Rab18-sfGFP(N) HDR template

   Plasmid

   #129415

   Purpose

   HDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   RAB18 HDR template (RAB18 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSK221

   Plasmid

   #129249

   Purpose

   reverse yTRAP, tetR-responsive Neon reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   pADH1(tetO2)-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-FGD1

   Plasmid

   #129612

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   FGD1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pN1-NLS-pHlu

   Plasmid

   #129320

   Purpose

   pH measurement, nucleus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   pHluorin (from H. Imamura)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV 3xFLAG-ATG3 WT

   Plasmid

   #129296

   Purpose

   Expresses 3xFLAG-ATG3 WT in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-PREX1

   Plasmid

   #129619

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   PREX1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pN1-NLS-QUE37C

   Plasmid

   #129323

   Purpose

   ATP measurement, nucleus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   QUEEN-37C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

    Plasmid

    #129722

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-3XFlag

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mNeongreen-aGBD(anillin)

    Plasmid

    #129633

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    RhoA binding domain of anillin (712-786)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-2T-RhoA-T19N

    Plasmid

    #12960

    Depositor

    Gary Bokoch

    Insert

    RhoA DN (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    T19N Dominant negative

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-myc-Cdc42-wt

    Plasmid

    #12972

    Depositor

    Gary Bokoch

    Insert

    Cdc42 (CDC42 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #129418

    Purpose

    Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    SpCas9 and sgRAB18.N (RAB18 Human)
    

    Use

    CRISPR

    Tags

    T2A-mCherry-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-myc-Rac1-wt

    Plasmid

    #12985

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBR5pemIKpKan

    Plasmid

    #129271

    Purpose

    Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

    Depositor

    Lindsey Burbank

    Article

    Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge