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Plasmid#61057PurposesfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A siteDepositorInsert24xGCN4_v1 and sfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK095
Plasmid#59560PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2DepositorInsertSGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-Ala11-GFP
Plasmid#126654PurposeInducible expression of Draper-Ala11-GFP mutant in S2 cellsDepositorInsertDraper cytoplasmi Tyr residues mutated to Ala
UseTagsExpressionInsectMutationCytoplasmic Tyr residues to AlaPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT Shark-TagBFP
Plasmid#126658PurposeInducible expression of Shark-TagBFP in S2 cellsDepositorInsertShark
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133b
Plasmid#103223PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133b target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133b target (MIR133B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro
Plasmid#60912PurposeEnocdes Camsap1L1 fused to the SunTag for single molecule imagingDepositorInsertCamsap1L1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
TLS 2: hTLS.pCDNA1
Plasmid#21828DepositorInsertTLS (FUS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTK296
Plasmid#59565PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PESTDepositorInsertSGP(14) 2xK-turn Kozak EGFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-BiP-Myc-KDEL P495L
Plasmid#27166DepositorInsertBiP (HSPA5 Human)
UseTagsKDEL and MycExpressionMammalianMutationPeptide Binding Mutant: P495LPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pHR KIF17 (1-738)
Plasmid#60406PurposeExpression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738DepositorInsertKIF17 (KIF17 Human)
UseLentiviralTagsGB1, Strep-Tag, and sfGFPExpressionMammalianMutationTruncated version amino acids 1-738PromoterSFFVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PERK 3: PERK.dC.9E10.pCDNA
Plasmid#21816DepositorInsertPERK (C-term deleted) (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationamino-acids 582-1081 deletedPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf (XSB522)
Plasmid#78512PurposeConstruct for expressing His10 - SNAPf in E. ColiDepositorInsertSNAPf
UseTagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BiP-Myc-KDEL T37G
Plasmid#27165DepositorInsertBiP (HSPA5 Human)
UseTagsKDEL and MycExpressionMammalianMutationATP bound form: T37GPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C147S_pQE30
Plasmid#48631PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion E147B, C147SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1-N100
Plasmid#19848DepositorInsertMKL1-N100 (MRTFA Human)
UseRetroviralTags3xFLAGExpressionMutationdeleted amino acids 1-100PromoterAvailable sinceJan. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pTK194
Plasmid#59563PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak mKate opal attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only