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Plasmid#92248PurposeHTT donor DNA with 45Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR81Q
Plasmid#92250PurposeHTT donor DNA with 81Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorTagsExpressionMutationBarcode of wobble bases to prevent TALEN cleaving…PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335 HTT sgRNA-a
Plasmid#87201PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTTDepositorInsertHTT (HTT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335 HTT sgRNA-b
Plasmid#87200PurposepX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTTDepositorInsertHTT (HTT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
LIC4B-His-TEV-TAK1-TAB1
Plasmid#154874PurposeInsect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91DepositorInsertTAK1-TAB1 (TAB1 Human)
UseTagsLIC TEV HisExpressionInsectMutationCodon optimizedPromoterpolyhedrinAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19_extendedTTE1564
Plasmid#60999PurposeT7-transcription template for Tte mRNA used in SiM-KARTSDepositorInsertTTE1564 transcript
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR11 mSTIM1 myc
Plasmid#17733DepositorInsertSTIM1 (Stim1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10
Plasmid#126948PurposeBacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mSTIM1 myc
Plasmid#17732DepositorInsertSTIM1 (Stim1 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)3
Plasmid#127150PurposeMammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AdEasier-1 cells (strain)
Bacterial Strain#16399DepositorBacterial ResistanceAmpicillin and streptomycinSpeciesEscherichia coliAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
Syn61Δ3(ev5) ΔrecA (ev1)
Bacterial Strain#189857PurposeLaboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.DepositorBacterial ResistanceStreptomycinSpeciesSyntheticAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAR TALE Assembly Kit
Plasmid Kit#1000000117PurposeThese plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.DepositorAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only