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pExo84-CRU 303

Plasmid

#131165

Purpose

For C-terminal genomic integration of Cub-R-URA3, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Insert

ScExo84

Tags

CRU

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-YFP-PKD1

Plasmid

#31527

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-HA-Protein Kinase D (PRKD1 Human)

Tags

HA and YFP

Expression

Mammalian

Available Since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-YFP-PKD2

Plasmid

#31821

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

Insert

YFP-Protein Kinase D2 (PRKD2 Human)

Tags

YFP

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
SFTPB Promoter reporter

Plasmid

#88868

Purpose

Surfactant Protein B Gene Promoter Reporter

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Surfactant protein B gene promoter (SFTPB Human)

Use

Luciferase

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-SNAP-TRIM21

Plasmid

#196181

Purpose

For in vitro transcription of SNAP-TRIM21 mRNA

Depositor

Binyam Mogessie

Article

Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

Insert

SNAP-TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCUP1pNuiHA kanMX CEN

Plasmid

#131168

Purpose

for ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiae

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRevLCav2

Plasmid

#60806

Purpose

used to make mutations in yeast REV3

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Tags

c-myc

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pKC530 pBABE EGFP-ZNF335

Plasmid

#192698

Purpose

Retroviral transgene expression of human ZNF335

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

ZNF335 (ZNF335 Human)

Use

Retroviral

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB4

Plasmid

#134688

Purpose

Expresses His6-AmmB4 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB4

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#134687

Purpose

Expresses His6-AmmB3 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB2

Plasmid

#134686

Purpose

Expresses His6-AmmB2 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB1

Plasmid

#134685

Purpose

Expresses His6-AmmB1 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFP-KASH

Plasmid

#154373

Purpose

Vector for Cre-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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(SUMO)10

Plasmid

#126948

Purpose

Bacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-EGFP-KASH

Plasmid

#154374

Purpose

Vector for Flp-dependent expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

EGFP-KASH

Use

AAV

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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(SUMO)5

Plasmid

#126949

Purpose

Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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